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Khaki M, Salmanian A H, Abtahi H, Ganji A, Mosayebi G. Mesenchymal Stem Cells Differentiate to Endothelial Cells Using Recombinant Vascular Endothelial Growth Factor –A. rbmb.net. 2018; 6 (2) :144-150
URL: http://rbmb.net/article-1-156-en.html

Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran-Department of Microbiology and Immunology, School of Medicine, Arak University of Medical Sciences, Arak, Iran.
Abstract:   (370 Views)

Background: Vascular endothelial growth factor-A (VEGF-A), an endothelial cell-specific mitogen produced by various cell types, plays important roles in cell differentiation and proliferation. In this study we investigated the effect of recombinant VEGF-A on differentiation of mesenchymal stem cells (MSCs) to endothelial cells (ECs).
Methods: VEGF-A was expressed in E. coli BL21 (DE3) and BL21 pLysS competent cells with the pET32a expression vector. Recombinant VEGF-A protein expression was verified by SDS-PAGE and western blotting. Mesenchymal stem cell differentiation to ECs in the presence of VEGF-A was evaluated by flow cytometry and fluorescence microscopy.
Results: Recombinant VEGF-A was produced in E. coli BL21 (DE3) cells at 0.8 mg/mL concentration. Expression of CD31 and CD 144 was significantly greater, while expression of CD90, CD73, and CD44 was significantly less, in MSCs treated with our recombinant VEGF-A than in those treated with the commercial protein (p < 0.05).
Conclusions: Recombinant VEGF-A expressed in a prokaryotic system can induce MSCs differentiation to ECs and can be used in research and likely therapeutic applications.

Full-Text [PDF 471 kb]   (94 Downloads)    
Type of Article: Original Article | Subject: Cell Biology
Received: 2017/04/3 | Accepted: 2017/04/21 | Published: 2017/10/1

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