Volume 8, Issue 2 (Vol.8 No.2 July 2019)
rbmb.net 2019, 8(2): 161-167 |
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Department of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Science. & Molecular and cell biology Research center, Faculty of Medicine, Mazandaran University of Medical sciences, Sari, Iran.
Abstract: (3363 Views)
Background: Staphylococcus aureus is predominant at sites of biomaterial-associated infection (BAI) and frequently infects hospitalized individuals.
Methods: The polysaccharide intercellular adhesin (PIA) and S. epidermidis rSesC protein, major macromolecules in biofilm formation, were purified under native conditions and cloned and expressed in a prokaryotic host.
Results: Purification of the macromolecules was confirmed by FTIR and Western blotting.
Conclusions: The S. epidermidis SesC protein and PIA were successfully purified. Both are considered as vaccine candidates.
Methods: The polysaccharide intercellular adhesin (PIA) and S. epidermidis rSesC protein, major macromolecules in biofilm formation, were purified under native conditions and cloned and expressed in a prokaryotic host.
Results: Purification of the macromolecules was confirmed by FTIR and Western blotting.
Conclusions: The S. epidermidis SesC protein and PIA were successfully purified. Both are considered as vaccine candidates.
Type of Article: Original Article |
Subject:
Molecular Biology
Received: 2018/08/29 | Accepted: 2018/09/9 | Published: 2019/09/14
Received: 2018/08/29 | Accepted: 2018/09/9 | Published: 2019/09/14
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