Volume 8, Issue 2 (Vol.8 No.2 July 2019)
rbmb.net 2019, 8(2): 200-207 |
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Hajar Yaghoobi 
, Hakim Azizi , Mehdi Banitalebi-Dehkordi , Fatemeh Mohammad Rezaei , Shahram Arsang-Jnag , Mohammad Taheri * , Soudeh Ghafouri-Fard
, Hakim Azizi , Mehdi Banitalebi-Dehkordi , Fatemeh Mohammad Rezaei , Shahram Arsang-Jnag , Mohammad Taheri * , Soudeh Ghafouri-Fard
Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Abstract: (3329 Views)
Background: The enzyme beta-secretase 1 (BACE1) and its antisense transcript (BACE1-AS) have been implicated in the pathogenesis of Alzheimer's disease. Moreover, several lines of evidence point to their contribution in tumorigenesis.
Methods: In the present study, we evaluated expression of BACE1 mRNA (BACE1) and BACE1-AS in 54 breast cancer tissues and 54 adjacent non-cancerous tissues (ANCTs) from the same patients using quantitative real-time PCR.
Results: BACE1 was significantly down-regulated in tumoral tissues compared with ANCTs, while BACE1-AS expression was not significantly different between tumoral tissues and ANCTs. The Bayesian Multilevel model showed a significant difference in BACE1 expression between stage 1 and 2 cancers after age-effect adjustments. BACE1-AS expression was significantly greater in ER-positive than in ER-negative samples (P=0.01). BACE1 and BACE1-AS expression were not correlated with patient ages in any sample sets.
Conclusions: Significant correlations were detected between expression of these genes in both tumoral tissues and ANCTs. The current study provides evidence for differential BACE1 expression in breast tissues and suggests further assessment of the role of BACE1 in the pathogenesis of cancer.
Methods: In the present study, we evaluated expression of BACE1 mRNA (BACE1) and BACE1-AS in 54 breast cancer tissues and 54 adjacent non-cancerous tissues (ANCTs) from the same patients using quantitative real-time PCR.
Results: BACE1 was significantly down-regulated in tumoral tissues compared with ANCTs, while BACE1-AS expression was not significantly different between tumoral tissues and ANCTs. The Bayesian Multilevel model showed a significant difference in BACE1 expression between stage 1 and 2 cancers after age-effect adjustments. BACE1-AS expression was significantly greater in ER-positive than in ER-negative samples (P=0.01). BACE1 and BACE1-AS expression were not correlated with patient ages in any sample sets.
Conclusions: Significant correlations were detected between expression of these genes in both tumoral tissues and ANCTs. The current study provides evidence for differential BACE1 expression in breast tissues and suggests further assessment of the role of BACE1 in the pathogenesis of cancer.
Type of Article: Original Article |
Subject:
Molecular Biology
Received: 2019/05/3 | Accepted: 2019/09/2 | Published: 2019/09/14
Received: 2019/05/3 | Accepted: 2019/09/2 | Published: 2019/09/14
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