Volume 8, Issue 3 (Vol.8 No.3 Oct 2019)
rbmb.net 2019, 8(3): 216-226 |
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Department of Biochemistry, Faculty of Pharmacy, Beni-Suef University, 62514, Beni-Suef, Egypt & Department of Pharmaceutics and Pharmaceutical Technology, College of Pharmacy, Mutah University, 61710, Al-Karak, Jordan.
Abstract: (1133 Views)
Background: Selenium is a mineral that showed both pro- and anti-oxidant activities in various disease models. In this study, we evaluated the anti-tumor effect of selenium against 1,2-dimethylhydrazine (DMH)-induced colorectal cancer in BALB/C mice and its effect on apoptosis and angiogenesis.
Methods: Colorectal cancer was induced by subcutaneous injection of DMH (20 mg/kg body weight) in BALB/C mice once weekly for 20 weeks. Selenium (200 mg/L) was given to DMH plus selenium-treated group in the drinking water for the next 3 months.
Results: The DMH plus selenium-treated group exhibited significantly lower expression of cloned caudal-type homebox gene -2 (CDX-2) and vascular endothelial growth factor (VEGF) but higher caspase-3 expression level at p<0.001 compared to the DMH-treated group. Moreover, a decrease in the reduced glutathione content and glutathione peroxidase activity but an increase in the malondialdehyde content were observed at p<0.001. Both macroscopic and microscopic examination of the colorectal tissues confirmed the results.
Conclusions: The anti-tumor effect of selenium against an induced colorectal cancer in mice is attributed to its pro-oxidant, anti-angiogenic and apoptotic effects.
Methods: Colorectal cancer was induced by subcutaneous injection of DMH (20 mg/kg body weight) in BALB/C mice once weekly for 20 weeks. Selenium (200 mg/L) was given to DMH plus selenium-treated group in the drinking water for the next 3 months.
Results: The DMH plus selenium-treated group exhibited significantly lower expression of cloned caudal-type homebox gene -2 (CDX-2) and vascular endothelial growth factor (VEGF) but higher caspase-3 expression level at p<0.001 compared to the DMH-treated group. Moreover, a decrease in the reduced glutathione content and glutathione peroxidase activity but an increase in the malondialdehyde content were observed at p<0.001. Both macroscopic and microscopic examination of the colorectal tissues confirmed the results.
Conclusions: The anti-tumor effect of selenium against an induced colorectal cancer in mice is attributed to its pro-oxidant, anti-angiogenic and apoptotic effects.
Type of Article: Original Article |
Subject:
Cell Biology
Received: 2019/08/1 | Accepted: 2019/08/6 | Published: 2020/01/24
Received: 2019/08/1 | Accepted: 2019/08/6 | Published: 2020/01/24