Volume 2, Number 2 (Vol.2 No.2 Apr 2014)                   rbmb.net 2014, 2(2): 82-87 | Back to browse issues page



PMID: 26989726
PMCID: PMC4757051

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Sankian M, Hajavi J, Moghadam M, Varasteh A. Identification and Molecular Characterization of the cDNA Encoding Cucumis melo Allergen, Cuc m 3, a Plant Pathogenesis-Related Protein. rbmb.net. 2014; 2 (2) :82-87
URL: http://rbmb.net/article-1-47-en.html

Allergy Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran.
Abstract:   (1033 Views)

Background: Melon (Cucumis melo) allergy is one of the most common food allergies, characterized by oral allergy syndrome. To date, two allergen molecules, Cuc m 1 and Cuc m 2, have been fully characterized in melon pulp, but there are few reports about the molecular characteristics of Cuc m 3.

Methods:The Cuc m 3 cDNA has been characterized by rapid amplification of cDNA ends (RACE), which revealed a 456 base-pair (bp) fragment encoding a 151-amino acid polypeptide with a predicted molecular mass of 16.97 kDa, and identified 79 and 178 bp untranslated sequences at the 5′ and 3´ ends, respectively.

Results: In silico analysis showed strong similarities between Cuc m 3 and other plant pathogen-related protein 1s from cucumber, grape, bell pepper, and tomato.

Conclusion: Here we report the identification and characterization of the Cuc m 3 cDNA, which will be utilized for further analyses of structural and allergenic features of this allergen.

Full-Text [PDF 309 kb]   (169 Downloads)    
Subject: Immunology
Received: 2016/08/21 | Accepted: 2016/08/21 | Published: 2016/08/21

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