Volume 3, Issue 2 (Vol.3 No.2 Apr 2015)                   rbmb.net 2015, 3(2): 51-55 | Back to browse issues page

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Al-balaghee S, Al-balaghee Z, Shabani A, Ghadam P, Bandehpour M, Askari Mehr A et al . Determination of Haptoglobin Genotype in an Iranian Population with Idiopathic Generalized Epilepsy. rbmb.net. 2015; 3 (2) :51-55
URL: http://rbmb.net/article-1-58-en.html
Cellular and Molecular Biology Research Centre, Shahid Beheshti University of Medical Sciences, Tehran, Iran - Biotechnology Department, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract:   (4495 Views)

Background: Haptoglobin (Hp) is a plasma α2-sialoglycoprotein that contains alpha and beta chains. It displays in three common phenotypes, Hp1-1, Hp2-1, and Hp2-2. Proteins expressed by polymorphic genes have grossly different molecular sizes resulting in different diffusion rates in the brain. Haptoglobin expressed by the Hp2-2 genotype has lower hemoglobin-binding capacity than Hp1-1 or Hp2-1 and is associated with idiopathic generalized epilepsy.

Methods: To determine polymorphism in haptoglobin genes in patients with idiopathic generalized tonic-clonic seizures, 42 men, 42 women, and 50 controls were selected for this study. Genomic DNA was extracted from blood and studied by polymerase chain reactions (PCR).

Results: The amplified fragments for the Hp1-1 and Hp2-2 genotypes were 1757 and 3481 base pairs (bp) respectively, and the Hp2-1 genotype had both fragments, in addition to a 349-bp fragment. The distribution of the three major Hp phenotypes in epilepsy patients was 28.6 (1-1), 38.1 (2-1), and 33.3% (2-2) in the men, and 31 (1-1), 40.5 (2-1), and 28.6% (2-2) in the women. The distribution of Hp genotypes in controls was 22 (1-1), 40 (2-1), and 38% (2-2).

Conclusion: We show that all Hp genotypes participate in idiopathic generalized epilepsy.

Keywords: Epilepsy, Haptoglobin, Iran
Full-Text [PDF 210 kb]   (1454 Downloads)    
Subject: Biochemistry
Received: 2014/07/15 | Accepted: 2014/10/22 | Published: 2015/04/30

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