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Yazdi M, Nafari A, Azadpour M, Alaee M, Hadipour Moradi F, Choghakhori R, et al . Protective Effects of Cinnamic Acid Against Hyperglycemia Induced Oxidative Stress and Inflammation in HepG2 Cells. rbmb.net 2023; 12 (1) :1-12
URL: http://rbmb.net/article-1-914-en.html
URL: http://rbmb.net/article-1-914-en.html
Mohammad Yazdi 
, Amirhossein Nafari , Mojgan Azadpour , Mahdi Alaee , Forouzan Hadipour Moradi , Razieh Choghakhori , Maryam Hormozi , Hassan Ahmadvand *
, Amirhossein Nafari , Mojgan Azadpour , Mahdi Alaee , Forouzan Hadipour Moradi , Razieh Choghakhori , Maryam Hormozi , Hassan Ahmadvand *
Department of Biochemistry, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran & Medicinal plants and natural products research center, Hamadan university of medical sciences, Hamadan, Iran.
Abstract: (1416 Views)
Background: Cinnamic acid, a phenylpropanoid acid, has been investigated as a potential alternative therapy for diabetes and its complications in some studies.
Methods: In the first stage, the viability of HepG2 cells at different concentrations of glucose and CA was assessed by MTT assay. Oxidative stress markers) CAT, GPx, GSH, and MDA) were measured spectrophotometrically. After RNA extraction, the effect of different concentrations of CA on the expression of DPP4 and inflammatory factors (IL-6, NF- κB) in HepG2 cells was assessed using real-time PCR.
Results: In HepG2 cells, CA increased catalase and glutathione peroxidase activity and GSH production in a dose-dependent manner in the presence of high glucose concentrations, with the greatest effect seen at a concentration of 75 mg/ml. Also, it reduced the amount of MDA in high-glucose HepG2 cells. Furthermore, CA decreased the expression of DPP4, NF- κB, and IL-6 genes in HepG2 cells in the presence of high glucose levels.
Conclusions: The results of our study indicated that CA reduced hyperglycemia-induced complications in HepG2 cells by decreasing inflammatory gene expression, including IL-6 and NF- κB and inhibiting the expression of DPP4, and limiting oxidative stress.
Methods: In the first stage, the viability of HepG2 cells at different concentrations of glucose and CA was assessed by MTT assay. Oxidative stress markers) CAT, GPx, GSH, and MDA) were measured spectrophotometrically. After RNA extraction, the effect of different concentrations of CA on the expression of DPP4 and inflammatory factors (IL-6, NF- κB) in HepG2 cells was assessed using real-time PCR.
Results: In HepG2 cells, CA increased catalase and glutathione peroxidase activity and GSH production in a dose-dependent manner in the presence of high glucose concentrations, with the greatest effect seen at a concentration of 75 mg/ml. Also, it reduced the amount of MDA in high-glucose HepG2 cells. Furthermore, CA decreased the expression of DPP4, NF- κB, and IL-6 genes in HepG2 cells in the presence of high glucose levels.
Conclusions: The results of our study indicated that CA reduced hyperglycemia-induced complications in HepG2 cells by decreasing inflammatory gene expression, including IL-6 and NF- κB and inhibiting the expression of DPP4, and limiting oxidative stress.
Type of Article: Original Article |
Subject:
Biochemistry
Received: 2022/04/13 | Accepted: 2022/04/21 | Published: 2023/08/15
Received: 2022/04/13 | Accepted: 2022/04/21 | Published: 2023/08/15
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