%0 Journal Article %A Al-Mousawi, Masar Riyadh Rashid %A Mohammed Ali, May %A Al-Khafaji, Noor Salman Kadhim %A Al-Dahmoshi, Hussein Oleiwi Muttaleb %T First Record of HAdV-D20 AmongKeratoconjunctivitis Patients in Iraq %J Reports of Biochemistry and Molecular Biology %V 11 %N 1 %U http://rbmb.net/article-1-822-en.html %R 10.52547/rbmb.11.1.176 %D 2022 %K HAdV-D8, HAdV-D20, HAdV-D37, conjunctivitis, Iraq, %X Background: Human Adenovirus species D (HAdV-D) was common human viral pathogen especially in eye infection, consists of several types of which HAdV-D8, -D19 and –D37 were common in eye infection. This study includes detection of HAdV-D types implicated in conjunctivitis based on L2 (Penton protein) gene similarity. Methods: Conjunctival swabs were collected from Keratoconjunctivitis patients as eye infection related to adenovirus. Viral nucleic acids were extracted and specific primer pairs for HAdV-D L2 gene (encoding for penton base protein) was used to amplify the target gene and only positive samples were sent to sequencing. Results: The results revealed that only 6 samples give positive results for L2 gene amplification and then sent for sequencing for L2 (penton protein) gene-based typing. The results show that 4 local isolates (S1, S2, S3, S6) were similar to HAdV-D8 and 2 local isolates (S4, S5) were similar to HAdVD20. Also the results display that the HAdV-37, prominent HAdV-D type of human eye infection, may be variant of HAdV-D20 due to that six variation were seen in S4and S5 local isolates nucleotide sequence in relation to HAdV-D37: T>C at position 14364, A>C at position 14411, T>C at position 14427, C>A at position 14448, G>A at position 14540 and T>C at position 14617, leading to only 2 amino acid change in resulted penton protein: T (Threonine) instead of K (Lysine) at position 204 and N (Asparagine) instead of D (Aspartic acid) at position 247. Conclusions: The current study concludes the possibility of implication of HAdV-D20 in eye infections especially conjunctivitis. %> http://rbmb.net/article-1-822-en.pdf %P 176-181 %& 176 %! HAdV-D20 in Keratoconjunctivitis %9 Original Article %L A-10-779-4 %+ Department of Biology, college of science, University of Babylon, Iraq. %G eng %@ 2322-3480 %[ 2022