Reports of Biochemistry and Molecular Biology
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10.61186/rbmb
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2020
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H19/Igf2 Expression and Methylation of Histone 3 in Mice Chimeric Blastocysts
زیست شناسی سلولی
Cell Biology
مقالات اصلی
Original Article
<div style="text-align: justify;"><strong>Background: </strong>Currently, the efficient production of chimeric mice and their survival are still challenging. Recent researches have indicated that preimplantation embryo culture media and manipulation lead to abnormal methylation of histone in the H19/Igf2 promotor region and consequently alter their gene expression pattern. This investigation was designed to evaluate the relationship between the methylation state of histone<br>
H3 and H19/Igf2 expression in mice chimeric blastocysts.<br>
<br>
<strong>Methods:</strong> Mouse 129/Sv embryonic stem cells (mESCs) expressing the green fluorescent protein (mESCs- GFP) were injected into the perivitelline space of 2.5 days post-coitis (dpc) embryos (C57BL/6) using a micromanipulator. H3K4 and H3K9 methylation, and H19 and Igf2 expression was measured by immunocytochemistry and q-PCR, respectively, in blastocysts.<br>
<br>
<strong>Results: </strong>Histone H3 trimethylation in H3K4 and H3K9 in chimeric blastocysts was significantly less and greater, respectively (p< 0.05), than in controls. H19 expression was significantly less (p< 0.05), while Igf2 expression was less, but not significantly so, in chimeric than in control blastocysts.<br>
<br>
<strong>Conclusions:</strong> Our results showed, that the alteration ofH3K4me3 and H3K9me3 methylation, change H19/Igf2 expression in chimeric blastocysts.</div>
Chimeric blastocysts, H19/Igf2, Histone 3 (H3) methylation.
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365
http://rbmb.net/browse.php?a_code=A-10-531-1&slc_lang=en&sid=1
Maryam
Salimi
10031947532846007780
10031947532846007780
No
Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Abolfazl
Shirazi
a.shirazi@ari.ir
10031947532846007781
10031947532846007781
Yes
Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran & Department of Gametes and Cloning, Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran.
Mohsen
Norouzian*
norozian93@gmail.com
10031947532846007782
10031947532846007782
No
Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Ameneh
Jafari
10031947532846007783
10031947532846007783
No
Student Research Committee, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Haleh
Edalatkhah
10031947532846007784
10031947532846007784
No
Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Maryam
Mehravar
10031947532846007785
10031947532846007785
No
Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Mohammad
Majidi
10031947532846007786
10031947532846007786
No
Food and Drug Laboratory Research Center, Food and Drug Organization, MOH & ME, Tehran, Iran.
Mohammad Mehdi
Mehrazar
10031947532846007787
10031947532846007787
No
Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.