Reports of Biochemistry and Molecular Biology
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10.61186/rbmb
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2022
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Organophosphate Resistance in Aedes aegypti: Study from Dengue Hemorrhagic Fever Endemic Subdistrict in Riau, Indonesia
بیوشیمی
Biochemistry
مقالات اصلی
Original Article
<div style="text-align: justify;"><em><strong>Background:</strong></em> Dengue hemorrhagic fever (DHF) is a significant health problem. The high number of cases requires preventions, including controlling the dengue vector, Aedes aegypti mosquito. One of the control methods is the use of insecticides containing organophosphate. This study aims to detect organophosphate resistance in Aedes aegypti from DHF endemic subdistrict, Riau, Indonesia by a sensitivity test of temephos and 5% malathion and measuring the activity of non-specific alpha and beta esterase enzymes.<br>
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<strong><em>Methods: </em></strong>This observational study determined Aedes aegypti resistance from larvae to adult in one DHF endemic subdistrict in Riau, Indonesia. The bioassay was used for temephos sensitivity of Aedes aegypti larvae. The LC99 value was analyzed using probit and compared with the diagnostic value from WHO. The WHO susceptibility test was conducted to determine 5% malathion resistance from adult mosquitoes. The mortality of less than 90% was declared as resistant. Measurement of alpha and beta esterase levels used Lee's microplate assay technique based on visual identification and absorbance value (AV).<br>
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<em><strong>Results:</strong></em> The results showed that Aedes aegypti were resistant to temephos. It also showed that adult mosquitoes were resistant to 5% malathion. Based on the alpha esterase activity test, it was found that most of the mosquitoes showed very sensitive meanwhile, based on the beta esterase activity test, most of the mosquitoes were moderate resistance.<br>
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<strong><em>Conclusions:</em></strong> This study suggests that Aedes aegypti population from DHF endemic subdistrict in Riau, Indonesia are indicated to develop resistance to organophosphate.</div>
Aedes aegypti, Dengue Hemorrhagic fever, Organophosphate, Resistance.
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596
http://rbmb.net/browse.php?a_code=A-10-709-1&slc_lang=en&sid=1
Suri Dwi
Lesmana
suri.dwi@lecturer.unri.ac.id
100319475328460012430
100319475328460012430
Yes
Department of Parasitology, Faculty of Medicine, Universitas Riau, Pekanbaru, Indonesia.
Esy
Maryanti
100319475328460012431
100319475328460012431
No
Department of Parasitology, Faculty of Medicine, Universitas Riau, Pekanbaru, Indonesia.
Elva
Susanty
100319475328460012432
100319475328460012432
No
Department of Parasitology, Faculty of Medicine, Universitas Riau, Pekanbaru, Indonesia.
Dedi
Afandi
100319475328460012433
100319475328460012433
No
Department of Forensic and Medicolegal, Faculty of Medicine, Universitas Riau, Pekanbaru, Indonesia.
Wida
Harmas
100319475328460012434
100319475328460012434
No
Faculty of Medicine, Universitas Riau, Pekanbaru, Indonesia.
Delly Noer
Octaviani
100319475328460012435
100319475328460012435
No
Faculty of Medicine, Universitas Riau, Pekanbaru, Indonesia.
Iskandar
Zulkarnain
100319475328460012436
100319475328460012436
No
Faculty of Medicine, Universitas Riau, Pekanbaru, Indonesia.
Muhammad Agung Bariq
Pratama
100319475328460012437
100319475328460012437
No
Faculty of Medicine, Universitas Riau, Pekanbaru, Indonesia.
Mislindawati
Mislindawati
100319475328460012438
100319475328460012438
No
Department of Parasitology, Faculty of Medicine, Universitas Riau, Pekanbaru, Indonesia.