Reports of Biochemistry and Molecular Biology
rbmb.net
Basic Sciences
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admin
2322-3480
2322-3480
10.61186/rbmb
en
jalali
1401
5
1
gregorian
2022
8
1
11
2
online
1
fulltext
en
Association of a Genetic Variant in the Cyclin-Dependent Kinase Inhibitor 2B with Risk of Pancreatic Cancer
زیست شناسی ملکولی
Molecular Biology
مقالات اصلی
Original Article
<div style="text-align: justify;"><strong><em>Background: </em></strong>Pancreatic cancer (PC) is among the most aggressive tumors with a poor prognosis, indicating the need for the identification of a novel prognostic biomarker for risk stratifications. Recent genome-wide association studies have demonstrated common genetic variants in a region on chromosome 9p21 associated with an increased risk of different malignancies.<br>
<br>
<strong><em>Methods:</em></strong> In the present study, we explore the possible relationship between genetic variant, rs10811661, and gene expression of CDKN2B in 75 pancreatic cancer patients, and 188 healthy individuals. DNAs were extracted and genotyping and gene expression were performed by TaqMan real-time PCR and RT-PCR, respectively. Logistic regression was used to assess the association between risk and genotypes, while the significant prognostic variables in the univariate analysis were included in multivariate analyses.<br>
<br>
<em><strong>Results:</strong></em> The patients with PDAC had a higher frequency of a TT genotype for rs10811661 than the control group. Also, PDAC patients with dominant genetic model, (TT + TC), was associated with increased risk of developing PDAC (OR= 14.71, 95% CI [1.96-110.35], p= 0.009). Moreover, patients with CC genotype had a higher expression of CDKN2B, in comparison with TT genotype.<br>
<br>
<strong><em>Conclusions:</em></strong> Our findings demonstrated that CDKN2A/B was associated with the risk of developing PDAC, supporting further investigations in the larger and multicenter setting to validate the potential value of this gene as an emerging marker for PDAC.</div>
CDKN2A/B, Rs10811661, Pancreatic cancer, Prognostic biomarker.
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http://rbmb.net/browse.php?a_code=A-10-969-1&slc_lang=en&sid=1
Newsha
Sardarzade
100319475328460012285
100319475328460012285
No
Surgical Oncology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Fatemeh
khojasteh-Leylakoohi
100319475328460012286
100319475328460012286
No
Metabolic syndrome Research center, Mashhad University of Medical Sciences, Mashhad, Iran & Basic Medical Sciences Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Sedigheh
Damavandi
100319475328460012287
100319475328460012287
No
Metabolic syndrome Research center, Mashhad University of Medical Sciences, Mashhad, Iran.
Ghazaleh
Khalili-Tanha
100319475328460012288
100319475328460012288
No
Basic Medical Sciences Institute, Mashhad University of Medical Sciences, Mashhad, Iran & Medical Genetics Research center, Mashhad University of Medical Sciences, Mashhad, Iran.
Mohammad
Dashtiahangar
100319475328460012289
100319475328460012289
No
Metabolic syndrome Research center, Mashhad University of Medical Sciences, Mashhad, Iran.
Nima
Khalili-Tanha
100319475328460012290
100319475328460012290
No
Metabolic syndrome Research center, Mashhad University of Medical Sciences, Mashhad, Iran.
Amir
Avan
100319475328460012291
100319475328460012291
No
Metabolic syndrome Research center, Mashhad University of Medical Sciences, Mashhad, Iran & Basic Medical Sciences Institute, Mashhad University of Medical Sciences, Mashhad, Iran & Medical Genetics Research center, Mashhad University of Medical Sciences, Mashhad, Iran & Department of Medical Genetics and Molecular Medicine, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Sakineh
Amoueian
100319475328460012292
100319475328460012292
No
Department of Pathology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Seyed Mahdi
Hassanian
100319475328460012293
100319475328460012293
No
Metabolic syndrome Research center, Mashhad University of Medical Sciences, Mashhad, Iran & Basic Medical Sciences Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Habibollah
Esmaily
100319475328460012294
100319475328460012294
No
Metabolic syndrome Research center, Mashhad University of Medical Sciences, Mashhad, Iran & Basic Medical Sciences Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Majid
Khazaei
100319475328460012295
100319475328460012295
No
Metabolic syndrome Research center, Mashhad University of Medical Sciences, Mashhad, Iran & Basic Medical Sciences Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Gordon
Ferns
100319475328460012296
100319475328460012296
No
Brighton & Sussex Medical School, Division of Medical Education, Falmer, Brighton, Sussex BN1 9PH, UK.
Alireza
Khooei
KhooeiAR@mums.ac.ir
100319475328460012297
100319475328460012297
No
Department of Pathology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Mohsen
Aliakbarian
AliakbarianM@mums.ac.ir
100319475328460012298
100319475328460012298
Yes
Surgical Oncology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.