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Antibacterial Efficacy of Walnut Green Husk (WGH) Extract with Zinc Oxide Nanoparticles on Streptococcus Mutans
میکروب شناسی
Microbiology
مقالات اصلی
Original Article
<span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Background:</span></span></i></b><span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> Chemical<b> </b>agents, such as Chlorhexidine are used as one of dental plaque control strategy. Researchers are looking for a natural and economic substitute with same antibacterial efficacy and less complications. The aim of this study was to evaluate the antimicrobial efficacy of the Khorasan Razavi walnut green husk extract with and without adding ZnO nanoparticles (nZnO) on <i> (S. mutans).</i></span></span><br>
<span style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span><br>
<span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Methods:</span></span></i></b><span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> In this <i>in vitro</i> study, antimicrobial effect of the Hydro-ethanolic extract of WGH, was evaluated. Broth Dilution and Agar diffusion methods were used with 90 tubes containing different dilutions of WGH extract (100 to 0.006 mg/ml). ZnO nanoparticles (nZnO) were added to 45 tubes. <i>Streptococcus mutans</i> was exposed to 15 different serial concentrations of study extracts, from 100 mg/ml to 0.006 mg/ml. Minimum inhibitory concentration (MIC) of the study extracts were determined and zone of inhibition diameter was compared to positive controls (chlorhexidine 0.2%, nZnO), and negative control (sterile distilled water). The differences between the mean diameters, were analyzed by independent sample T- teS.</span></span></span></span></span></span><br>
<br>
<span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></i></b><span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> Minimum inhibitory concentration (MIC) of study extract was found to be 50 mg/mL, with adding nZnO, MIC was reduced to 3.12 mg/mL. Mean diameter of inhibition zone at 3.12 mg/ml with and without adding ZnO nanoparticles were 17.67±0.57 mm and 8±0.001 mm, respectively, (</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">p</span></span><span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">-value<</span></span> <span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">0.001).</span></span></span></span></span></span><br>
<br>
<span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Conclusions:</span></span></i></b> <span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Adding nZnO could be enhanced antimicrobial efficacy of the WGH extract against <i>S. mutants</i>, while it was still less effective than chlorhexidine.</span></span></span></span></span></span>
Dental decay, Nanoparticles, Streptococcus mutans, Walnut green husk, Zinc oxide.
405
410
http://rbmb.net/browse.php?a_code=A-10-987-1&slc_lang=en&sid=1
Taraneh
Movahhed
100319475328460015542
100319475328460015542
No
Dental School, Mashhad University of Medical Sciences, Iran.
Maryam
Mehrabkhani
100319475328460015543
100319475328460015543
No
Dental School, Mashhad University of Medical Sciences, Iran.
Mohsen
Arefnezhad
100319475328460015544
100319475328460015544
No
Kashmar School of Nursing, Mashhad University of Medical Sciences, Iran.
Shokouh Sadat
HAmedi
100319475328460015545
100319475328460015545
No
School of Persian and complementary medicine, Mashhad University of Medical Sciences, Iran.
Reza
Zare Mahmoudabadi
100319475328460015546
100319475328460015546
No
Dental School, Mashhad University of Medical Sciences, Iran.
Fariba
Ghanbari
Ghanbarif981@mums.ac.ir
100319475328460015547
100319475328460015547
Yes
Dental School, Mashhad University of Medical Sciences, Iran.
Mahjube
Rostami
100319475328460015548
100319475328460015548
No
Dental School, Mashhad University of Medical Sciences, Iran.