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In Vitro Cytotoxic Activity of Total Flavonoid from Equisetum Arvense Extract
بیوشیمی
Biochemistry
مقالات اصلی
Original Article
<div style="text-align: justify;"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span lang="EN-CA" style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">Background:</span></span></span></i></b> <span style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">Normally happening substances like flavonoids are regarded as active candidates for the treatment and prevention of cancer </span></span></span><span style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">The purpose of this study was to see how Iraqi <i>E. arvense</i> total flavonoid affected cell lines biologically and human lung fibroblast normal cell line (WISH).</span></span></span></span></span></span></span><br>
<br>
<span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span lang="EN-CA" style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">Methods:</span></span></span></i></b> <span style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:.2pt">Plant powder was extracted by reflex apparatus, then thin-layer chromatography (TLC) was used to determine total flavonoids. Cytotoxicity assay (MTT) was used to determine the cytotoxic activity of the prepared plant against human breast cancer (MCF-7), cells human cervix cancer (HELA), human colon cancer (Caco-2) and human lung fibroblast normal cell line (WISH).</span></span></span></span><br>
<span style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"></span></span></span></span></span></span></span><br>
<span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span lang="EN-CA" style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">Results:</span></span></span></i></b> <span style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">The flavonoids Rutin, Quercetin, Kaempferol, and luteolin were detected using the Thin Layer Chromatography (TLC) technique. In contrast to the negative control, the extract inhibited cell growth to a highest of 82.158% for MCF-7 and 61.360% for Caco-2 at the concentration (100 µg/ml), and (54.880%) for Hela cell line at the concentration (100 µg/ml). In addition, the concentration (6.25 µg/ml) of total flavonoid extract produced a decrease in the growth of the normal WISH cell line to reach (1.094%).</span></span></span></span></span></span></span><br>
</div>
<div style="text-align: justify;"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span lang="EN-CA" style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">Conclusions:</span></span></span></i></b> <i><span style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">Equisetum arvense</span></span></span></i><span style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"> contain high amounts of flavonoids, the qualification of some flavonoids compounds was detected using TLC. The total flavonoids showed significant cytotoxic activity against various types of cancer cell lines and normal cell line <i>in vitro</i>, the antitumor activity was highly efficient in a dose and cell type dependent manner</span></span></span></span></span></span></span></div>
Keywords: Equisetum arvense L, Caco2, Hela, Total flavonoid, MCF-7, WISH.
487
492
http://rbmb.net/browse.php?a_code=A-10-986-1&slc_lang=en&sid=1
Raghda
Makia
raghdahmakia@gmail.com
100319475328460013452
100319475328460013452
Yes
Biotechnology College, Al-Nahrain University Baghadad, Iraq.
Khulood
Al-Sammarrae
100319475328460013453
100319475328460013453
No
Department of Forensic Techniques, Al-Farahidi University, Baghadad, Iraq.
Mohammad
Al-Halbosiy
100319475328460013454
100319475328460013454
No
Biotechnology Research Center, Al-Nahrain University, Baghadad, Iraq.
Mohammed
Al-Mashhadani
100319475328460013455
100319475328460013455
No
Department of Chemistry, College of Science, Al-Nahrain University, Baghdad, Iraq.