Reports of Biochemistry
and Molecular Biology
Fri, Apr 19, 2024
[Archive]
Volume 10, Issue 1 (Vol.10 No.1 Apr 2021)
rbmb.net 2021, 10(1): 41-49 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Reastuty R, Haryuna T S H. Correlation of SOD and MDA Expression in the Organ of Corti and Changes in the Function of Outer Hair Cells Measured by DPOAE Examination in Noise-Exposed Rat Cochlea. rbmb.net 2021; 10 (1) :41-49
URL: http://rbmb.net/article-1-604-en.html
URL: http://rbmb.net/article-1-604-en.html
Department of Otorhinolaryngology, Faculty of medicine, Universitas Sumatera Utara, 20155, Medan, North Sumatera, Indonesia.
Abstract: (2949 Views)
Background: Hearing loss due to noise can cause the disturbances toward the quality of life and cause mechanical damage and metabolic decompensation. Distortion Product Otoacoustic Emission (DPOAE) is an examination to assess the sensory function of outer hair cells. Superoxide Dismutase (SOD) and Malondialdehyde (MDA) are markers of oxidative stress. The aim of this study was to identify the correlation between DPOAE examination and SOD and MDA expression in rats exposed to noise.
Methods: This research was conducted on 27 rats which were divided into 3 groups, group 1 (control), group 2, and group 3 were groups with 100 dB and 110 dB noise exposure respectively.
Results: Our findings show a decrease in SOD expression and DPOAE values as well as an increase in MDA expression in rats exposed to noise and there is a positive correlation between Signal to Noise Ratio (SNR) value with SOD expression (r= 0.733, p= 0.025) and a negative correlation between SNR value with MDA expression (r= -0.678, p= 0.045).
Conclusions: our study find the correlation of oxidant and antioxidant status values in the organ of corti and changes in the function of outer hair cells in noise-exposed rat models.
Methods: This research was conducted on 27 rats which were divided into 3 groups, group 1 (control), group 2, and group 3 were groups with 100 dB and 110 dB noise exposure respectively.
Results: Our findings show a decrease in SOD expression and DPOAE values as well as an increase in MDA expression in rats exposed to noise and there is a positive correlation between Signal to Noise Ratio (SNR) value with SOD expression (r= 0.733, p= 0.025) and a negative correlation between SNR value with MDA expression (r= -0.678, p= 0.045).
Conclusions: our study find the correlation of oxidant and antioxidant status values in the organ of corti and changes in the function of outer hair cells in noise-exposed rat models.
Type of Article: Original Article |
Subject:
Molecular Biology
Received: 2020/11/10 | Accepted: 2020/12/22 | Published: 2021/05/9
Received: 2020/11/10 | Accepted: 2020/12/22 | Published: 2021/05/9
References
1. Hong O, Kerr MJ, Poling GL, Dhar S. Understanding and preventing noise induced hearing loss. Dis Mon. 2013;59(4):110-8 [DOI:10.1016/j.disamonth.2013.01.002] [PMID]
2. Basner M, Babisch W, Davis A, Brink M, Clark C, Janssen S, et al. Auditory and non-auditory effects of noise on health. Lancet. 2014;383(9925):1325-1332. [DOI:10.1016/S0140-6736(13)61613-X]
3. Mathur NN. Noise induced hearing loss emedicine background, pathophysiology, epidemiology. 2020. Available from: https://emedicine.medscape.com/article/857813-overview.
4. Kowalska M, Davis A. Noise-induced hearing loss. Noise & Health. 2012;14(61):274-80. [DOI:10.4103/1463-1741.104893] [PMID]
5. World Health Organization Determination of risk of noise-induced hearing loss due to recreational sound: review [web page on the internet]. 2017 [cited 2020 Nov 25]. Available from:https://www.who.int/pbd/deafness/Monograph_on_determination_of_risk_of_HL_due_to_exposure_to_recreational_sounds.pdf.
6. Stucken EZ, Hong RS. Noise-induced hearing loss: an occupational medicine perspective. Curr Opin Otolaryngol Head Neck Surg. 2014;22(5):388-93. [DOI:10.1097/MOO.0000000000000079] [PMID]
7. Kurabi A, Keithley EM, Housley GD, Ryan AF, Wong ACY. Cellular mechanisms of noise-induced hearing loss. Hear Res. 2017;349:129-137. [DOI:10.1016/j.heares.2016.11.013] [PMID] [PMCID]
8. Choi CH. Mechanisms of noise-induced hearing loss and treatment. Audiology Speech Res. 2011;7:124-32. [DOI:10.21848/audiol.2011.7.2.124]
9. World Health Organization. Hearing loss due to recreational exposure to loud sounds: A Review. WHO. 2015. Available from: https://apps.who.int/iris/bitstream/handle/10665/154589/9789241508513_eng.pdf?sequence=1&isAllowed=y
10. Zimatore G, Stanzial D, Orlando MP. Otoacoustic Emissions. In: Research and Applications. In-Tech, London 2013;204-23. [DOI:10.5772/55254] [PMCID]
11. Kemp DT. Otoacoustic emissions. In Encyclopedia of neuroscience. Centre for Auditory Research Elsevier. 2009;317-36. [DOI:10.1016/B978-008045046-9.00265-5]
12. Saurini P, Nola G, Lendvai D. Otoacoustic emissions: a new method for newborn hearing screening. Eur Rev Med Pharma Sci. 2004; 8:129-33.
13. Yuan H, Wang X, Hill K, Chen J, Lemasters J, Yang SM, et al. Autophagy attenuates noise-induced hearing loss by reducing oxidative stress. Antioxid Redox Signal. 2015;22(15):1308-24. [DOI:10.1089/ars.2014.6004] [PMID] [PMCID]
14. Pinar T, Atli AK, Alacam H, Karabulu I, Soguksulu I, Atas A, et al. The effects of noise on oxidative and antioxidative balance in human erythrocytes. Int J Hem Onc. 2011;(21):10-18. [DOI:10.4999/uhod.10094]
15. National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals. Guide for the Care and Use of Laboratory Animals. 8th ed. Washington (DC): National Academies Press (US); 2011.
16. Animal Care and Use Program. Rat and Mouse anesthesia and analgesia: Formulary and General Drug Information. The University of British Columbia; 2016. https://animalcare.ubc.ca/sites/default/files/documents/Guideline%20-%20Rodent%20Anesthesia%20Analgesia%20Formulary%20%282016%29.pdf
17. Toydemir T, Kanter M, Erboga M, Oguz S, Erenoglu C. Antioxidative, antiapoptotic and proliferative effect of curcumin on liver regeneration after partial hepatectomy in rats. Toxicol Ind Health. 2015;31(2):162-72. [DOI:10.1177/0748233712469658] [PMID]
18. Haryuna TSH, Purba AH. W, Farhat F, Putra ST. The effect of Curcumin as an antioxidant on cochlea fibroblasts in ototoxic rat models. Journal of Chinese Pharmaceutical Sciences. 2018;27(12):847-854. [DOI:10.5246/jcps.2018.12.086]
19. Czogalla B, Kahaly M, Mayr D, Schmoekel E, Niesler B, Kolben T, et al. Interaction of ERα and NRF2 impacts survival in ovarian cancer Patients. Int J Mol Sci. 2019;20(1):112. [DOI:10.3390/ijms20010112] [PMID] [PMCID]
20. Salvi R, Boettcher FA. Animal Models of Noise-Induced Hearing Loss. Sourcebook of Models for Biomedical Research. 2008;289-301. [DOI:10.1007/978-1-59745-285-4_32]
21. Millon B, Mitra S, Song Y, Marguilles Z, Casserly R, Drake V, et al. The impact of biological sex on the response to nois and of otoprotective therapies against acoustic injury in mice. Biol Sex Differ. 2018;9(1):12. [DOI:10.1186/s13293-018-0171-0] [PMID] [PMCID]
22. Heffner H. Hearing in glires: domestic rabbit, cotton rat, feral house mouse, and kangaroo rat. Journal of the Acoustical Society of America. 1980;68(6):1584-99. [DOI:10.1121/1.385213]
23. Haryuna TSH, Riawan W, Nasution A, Ma'at S, Harahap J, Adriztina I. Curcumin reduces the noise-exposed cochlear fibroblasts apoptosis. Int Arch Otorhinolaryngol. 2016;20(4):370-376. [DOI:10.1055/s-0036-1579742] [PMID] [PMCID]
24. Bielefeld EC, Hu BH, Harris KC, Henderson D. Damage and threshold shift resulting from cochlear exposure to Paraquat-generated superoxide. Hear Res. 2005;207(1-2):35-42. [DOI:10.1016/j.heares.2005.03.025] [PMID] [PMCID]
25. Poirrier AL, Pincemail J, Van Den Ackerveken P, Lefebvre PP, Malgrange B. Oxidative stress in the cochlea: An update. Curr Med Chem. 2010;17(30):3591-604. [DOI:10.2174/092986710792927895] [PMID]
26. Henderson D, Hu B, Bielefeld E. Patterns and mechanisms of noise-induced cochlear pathology. Springer. 2008;31:195-217. [DOI:10.1007/978-0-387-72561-1_7]
27. Demirel R, Mollaoğlu H, Yeşilyurt H, Üçok K, Ayçiçek A, Akkaya M, et al. Noise induces oxidative stress in rat. Eur J Gen Med. 2009;6(1):20-4. [DOI:10.29333/ejgm/82631]
28. Xiong M, Lai H, Yang C, Huang W, Wang J, Fu X, et al. Comparison of the protective effects of radix astragali, α-lipolic acid, and vitamin E on accute acouctic trauma. Clin Med Insights Ear Nose Throat. 2012;5:25-31. [DOI:10.4137/CMENT.S10711] [PMID] [PMCID]
29. Deghani A, Ranjbarian M, Khavanin A, Rezazade-Azari M, Vosooghi S. Expossure to noise pollution and its effect on oxidants and antioxidant parameter in blood and liver tissue of rat. Zahedan Journal Research in Medical Sciences. 2013;15(5):13-17.
30. Nasezadeh P, Shahi F, Fridoni M, Seydi E, Izadi M, Salimi A. Moderate O3/O2 therapy enhances enzymatic andnnon-enzymatic antioxidant in brain and cochlear that protects noise-induced hearing loss. Free Radic Res. 2017;51(9-10):828-837. [DOI:10.1080/10715762.2017.1381695] [PMID]
31. Tuerdi A, Kinoshitaa M, Kamogashiraa T, Fujimotoa C, Iwasakia S, Shimizub T, et al. Manganese superoxide dismutase influences the extent of noise-induced hearing loss in mice. Neurosci Lett. 2017;642:123-128. [DOI:10.1016/j.neulet.2017.02.003] [PMID]
32. Abdel-Tawab MS, Tork OM, Mostafa-Hedeab G, Ewaiss-Hassan M. Protective Effects of Quercetin and Melatonin on Indomethacin Induced Gastric Ulcers in Rats. Rep Biochem Mol Biol. 2020;9(3):278-90. [DOI:10.29252/rbmb.9.3.278] [PMID] [PMCID]
33. Cheraghi M, Ahmadvand H, Maleki A, Babaeenezhad E, Shakiba S, Hassanzadeh F. Oxidative Stress Status and Liver Markers in Coronary Heart Disease. Rep Biochem Mol Biol. 2019;8(1):49-55.
34. Nandi SS, Dhatrak SV. Occupational noise-induced hearing loss in India. Indian J Occup Environ Med. 2008;12(2):53-56. [DOI:10.4103/0019-5278.43260] [PMID] [PMCID]
35. Nassiri P, Zare S, EsmaeelPour MRM, Pourbakht A, Azam K, Golmohammadi T. Assessment of the Effects of Different Sound Pressure Levels on Distortion Product Otoacoustic Emissions (DPOAEs) in Rats. International Journal of Occupational Hygiene. 2016;8(2):93-9.
36. Derekoy FS, Dundar Y, Aslan R, Cangal A. Influence of noise exposure on antioxidant system and TEOAEs in rabbits. Eur Arch Otorhinolaryngol. 2001;258(10):518-22. [DOI:10.1007/s004050100388] [PMID]
37. Henderson D, Bielefeld EC, Harris KC, Hu BH. The role of oxidative stress in noise-induced hearing loss. Ear Hear. 2006;27(1):1-19. [DOI:10.1097/01.aud.0000191942.36672.f3] [PMID]
38. Yildirim I, Kilinc M, Okur E, Inanc TF, Kilic MA, Kurutas EB, et al. The effects of noise on hearing and oxidative stress in textile workers. Ind Health. 2007;45(6):743-9. [DOI:10.2486/indhealth.45.743] [PMID]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
