Volume 10, Issue 3 (Vol.10 No.3 Oct 2021)                   rbmb.net 2021, 10(3): 354-361 | Back to browse issues page

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Rezaee H, Abbasnia S, Alenabi A, Vakili R, Moheghi N, Tavakol Afshari J et al . Expression of Vascular Endothelial Growth Factor A and Its Type 1 Receptor in Supratentorial Neoplasm. rbmb.net. 2021; 10 (3) :354-361
URL: http://rbmb.net/article-1-681-en.html
Immunology Research Centre, Inflammation and inflammatory Diseases division, Mashhad University of Medical Sciences, Mashhad, Iran.
Abstract:   (504 Views)
Background: Vascular endothelial growth factor (VEGF) is one of the main angiogenesis regulators in solid cancers. The brain solid tumors are life threatening diseases in which angiogenesis is an important phase of development and progression.
In the present study the gene expression of VEGF-A and VEGF receptor (VEGF-R1) were evaluated in CNS brain tumors.
Methods: The quantification of the VEGF-A and VEGF-R1 expressions was carried out by real-time PCR on fresh biopsies of 38 supratentorial brain tumors compared to 30 non-tumoral tissues. Then the correlations were investigated with clinic-pathological and demographic factors of the patients.
Results: PCR products sequencing confirmed the validity of the qRT-PCR. Although, VEGF-A and VEGF-R1 expression showed increased trends with progression of cell proliferation in different stages of astrocytoma (p=0.006,p=0.07, respectively), in case of VEGF-R1 did not met 95% confidence interval in other brain tumors. An increasing trend in VEGF-A and a declining trend in VEGF-R1 expression from stage-I to II were observed in meningioma.VEGF-A and VEGF-R1 expressions had not significant correlation with age and gender. Although, peritumoral brain edema (PTBE) in astrocytoma was significantly associated with tumor stages (p=0.01), VEGF-A and VEGF-R1 had not associations with PTBE in meningioma and metastasis.
Conclusion: VEGF-A is a valuable factor for prognosis of PTBE and malignancy stage in astrocytoma, and useful for monitoring of the treatment approaches.
Full-Text [PDF 299 kb]   (245 Downloads)    
Type of Article: Original Article | Subject: Immunology
Received: 2021/04/8 | Accepted: 2021/04/20 | Published: 2021/12/5

1. Dunn IF, Heese O, Black PM. Growth factors in glioma angiogenesis: FGFs, PDGF, EGF, and TGFs. J Neurooncol. 2000;50(1-2):121-37. [DOI:10.1023/A:1006436624862] [PMID]
2. Klagsbrun M, D'Amore PA. Vascular endothelial growth factor and its receptors. Cytokine Growth Factor Rev. 1996;7(3):259-70. [DOI:10.1016/S1359-6101(96)00027-5]
3. Stummer W. Mechanisms of tumor-related brain edema. Neurosurg Focus. 2007;22(5):E8. [DOI:10.3171/foc.2007.22.5.9] [PMID]
4. Dasararaju R, Mehta A. Current advances in understanding and managing secondary brain metastasis. CNS oncology. 2013;2(1):75-85. [DOI:10.2217/cns.12.33] [PMID] [PMCID]
5. Stockhammer G, Obwegeser A, Kostron H, Schumacher P, Muigg A, Felber S, et al. Vascular endothelial growth factor (VEGF) is elevated in brain tumor cysts and correlates with tumor progression. Acta Neuropathol. 2000;100(1):101-5. [DOI:10.1007/s004010051199] [PMID]
6. Huang H, Held-Feindt J, Buhl R, Mehdorn HM, Mentlein R. Expression of VEGF and its receptors in different brain tumors. Neurol Res. 2005;27(4):371-7. [DOI:10.1179/016164105X39833] [PMID]
7. Xiang F, Tanaka J, Takahashi J, Fukuda T. Expression of vascular endothelial growth factor (VEGF) and its two receptors in diffusely infiltrating astrocytomas and relationship to proliferative activity of tumor cells. Brain tumor pathology. 2001;18(2):67-71. [DOI:10.1007/BF02479418] [PMID]
8. Hou J, Kshettry VR, Selman WR, Bambakidis NC. Peritumoral brain edema in intracranial meningiomas: the emergence of vascular endothelial growth factor-directed therapy. Neurosurgical focus. 2013;35(6):E2. [DOI:10.3171/2013.8.FOCUS13301] [PMID]
9. Azimi-Nezhad M. Vascular endothelial growth factor from embryonic status to cardiovascular pathology. Reports of biochemistry & molecular biology. 2014;2(2):59-69.
10. Dharmalingam P, Roopesh Kumar VR, Verma SK. Vascular endothelial growth factor expression and angiogenesis in various grades and subtypes of meningioma. Indian J Pathol Microbiol. 2013;56(4):349-54. [DOI:10.4103/0377-4929.125286] [PMID]
11. Yoshioka H, Hama S, Taniguchi E, Sugiyama K, Arita K, Kurisu K. Peritumoral brain edema associated with meningioma: influence of vascular endothelial growth factor expression and vascular blood supply. Cancer. 1999;85(4):936-44. https://doi.org/10.1002/(SICI)1097-0142(19990215)85:4<936::AID-CNCR23>3.0.CO;2-J [DOI:10.1002/(SICI)1097-0142(19990215)85:43.0.CO;2-J]
12. Sahana KR, Akila P, Prashant V, Chandra BS, Suma MN. Quantitation of vascular endothelial growth factor and interleukin-6 in different stages of breast cancer. Rep Biochem Mol Biol. 2017;6(1):33-39.
13. Lamszus K, Ulbricht U, Matschke J, Brockmann MA, Fillbrandt R, Westphal M. Levels of soluble vascular endothelial growth factor (VEGF) receptor 1 in astrocytic tumors and its relation to malignancy, vascularity, and VEGF-A. Clin Cancer Res. 2003;9(4):1399-405.
14. Fukumura D, Xu L, Chen Y, Gohongi T, Seed B, Jain RK. Hypoxia and acidosis independently up-regulate vascular endothelial growth factor transcription in brain tumors in vivo. Cancer Res. 2001;61(16):6020-4.
15. Schoch HJ, Fischer S, Marti HH. Hypoxia-induced vascular endothelial growth factor expression causes vascular leakage in the brain. Brain. 2002;125(Pt 11):2549-57. [DOI:10.1093/brain/awf257] [PMID]
16. Hlobilkova A, Ehrmann J, Knizetova P, Krejci V, Kalita O, Kolar Z. Analysis of VEGF, Flt-1, Flk-1, nestin and MMP-9 in relation to astrocytoma pathogenesis and progression. Neoplasma. 2009;56(4):284-90. [DOI:10.4149/neo_2009_04_284] [PMID]
17. Shibuya M. Vascular endothelial growth factor (VEGF) and its receptor (VEGFR) signaling in angiogenesis: a crucial target for anti-and pro-angiogenic therapies. Genes Cancer. 2011;2(12):1097-105. [DOI:10.1177/1947601911423031] [PMID] [PMCID]
18. Dickinson PJ, Roberts BN, Higgins RJ, Leutenegger CM, Bollen AW, Kass PH, et al. Expression of receptor tyrosine kinases VEGFR-1 (FLT-1), VEGFR-2 (KDR), EGFR-1, PDGFRalpha and c-Met in canine primary brain tumours. Vet Comp Oncol. 2006;4(3):132-40. [DOI:10.1111/j.1476-5829.2006.00101.x] [PMID]
19. Jin Kim Y, Hyun Kim C, Hwan Cheong J, Min Kim J. Relationship between expression of vascular endothelial growth factor and intratumoral hemorrhage in human pituitary adenomas. Tumori. 2011;97(5):639-46. [DOI:10.1177/030089161109700517] [PMID]
20. Yamasaki F, Yoshioka H, Hama S, Sugiyama K, Arita K, Kurisu K. Recurrence of meningiomas. Cancer. 2000;89(5):1102-10. https://doi.org/10.1002/1097-0142(20000901)89:5<1102::AID-CNCR20>3.0.CO;2-L [DOI:10.1002/1097-0142(20000901)89:53.0.CO;2-L]
21. Ding YS, Wang HD, Tang K, Hu ZG, Jin W, Yan W. Expression of vascular endothelial growth factor in human meningiomas and peritumoral brain areas. Ann Clin Lab Sci. 2008;38(4):344-51.
22. Paek SH, Kim CY, Kim YY, Park IA, Kim MS, Kim DG, et al. Correlation of clinical and biological parameters with peritumoral edema in meningioma. J Neurooncol. 2002;60(3):235-45. [DOI:10.1023/A:1021186401522] [PMID]
23. Pistolesi S, Fontanini G, Camacci T, De Ieso K, Boldrini L, Lupi G, et al. Meningioma-associated brain oedema: the role of angiogenic factors and pial blood supply. J Neurooncol. 2002;60(2):159-64. [DOI:10.1023/A:1020624119944] [PMID]
24. Reszec J, Hermanowicz A, Rutkowski R, Turek G, Mariak Z, Chyczewski L. Expression of MMP-9 and VEGF in meningiomas and their correlation with peritumoral brain edema. Biomed Res Int. 2015;2015:646853. [DOI:10.1155/2015/646853] [PMID] [PMCID]
25. Berkman RA, Merrill MJ, Reinhold WC, Monacci WT, Saxena A, Clark WC, et al. Expression of the vascular permeability factor/vascular endothelial growth factor gene in central nervous system neoplasms. J Clin Invest. 1993;91(1):153-9. [DOI:10.1172/JCI116165] [PMID] [PMCID]

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