Atefeh Sohan Forooshan Moghadam, Mitra Ataei, Ghazaleh Arabzadeh, Kowsar Falahati, Fatemeh Roshani, Mohammad Hossein Sanati,
Volume 8, Issue 4 (Vol.8 No.4 Jan 2020)
Abstract
Background: In multiple sclerosis (MS), the immune system acts against myelin lesions of the central nervous system, destroying neuronal fibers resulting in signal transmission disturbances in the nervous system. MicroRNAs play important roles in the post-transcriptional regulation of gene expression and in the regulation of disease activity and its response to treatment. The goal of this study was to determine the role of miR-18a-5p by comparing its expression in MS patients and healthy subjects.
Methods: RNA was isolated from blood samples of 32 MS patients and 32 healthy individuals, and miR-18a-5p expression was determined by real-time polymerase chain reaction (real-time PCR).
Results: miR-18a-5p expression was significantly less in MS patients than in healthy subjects.
Conclusions: The reduction of miR-18a-5p expression may be via pathway signaling. Altered signaling plays an important role in MS pathogenesis and the miR-18a-5p expression profile in blood cells can be described as a prognostic biomarker and identifier of high-risk individuals in MS.
Fatemeh Roshani, Hossien Delavar Kasmaee, Kowsar Falahati, Ghazaleh Arabzadeh, Atefeh Aohan Forooshan Moghadam, Mohammd Hossein Sanati,
Volume 10, Issue 3 (Vol.10 No.3 Oct 2021)
Abstract
Background: Etiology of multiple sclerosis is non-clarified. It seems that environmental factors impact epigenetic in this disease. Micro-RNAs (MIR) as epigenetic factors are one of the most important factors in non-genetically neurodegenerative diseases. It has been found MIR-144 plays a main role in the regulation of many processes in the central nervous system. Here, we aimed to investigation of MIR-144 expression alteration in Multiple sclerosis (MS) patients.
Methods: In this study 32 healthy and 32 MS patient's blood sample were analyzed by quantitative Real-Time PCR method and obtained data analyzed by REST 2009 software.
Results: Analysis of Real-Time PCR data revealed that miR-144 Increase significantly in MS patients compared to healthy controls. Conclusions: The increase of MIR-144 expression in MS patients is obvious. MIR-144 can be used as a biomarker of MS and help to early diagnosis and treatment of this disease.
