TY - JOUR JF - rbmb JO - rbmb.net VL - 10 IS - 4 PY - 2022 Y1 - 2022/1/01 TI - Evaluation of Radiotherapy on miR-374 Gene Expression in Colorectal Cancer Patient Blood Samples TT - N2 - Background: Current cancer treatments include surgery, radiotherapy, chemotherapy, and immunotherapy. Despite these treatments, a main issue in cancer treatment is early detection. microRNAs (miRNAs) can be used as markers to diagnose and treat cancers. This study investigated the effect of radiotherapy on miR-374 expression, and APC and GSK-3β, two of its target genes, in the WNT pathway, in peripheral blood samples from radiotherapy-treated colorectal cancer (CRC) patients. Methods: Peripheral blood was collected from 25 patients before and after radiotherapy. RNA was extracted from the blood and cDNA synthesized. miR-374, APC, and GSK-3β expression was determined by real-time polymerase chain reaction (RT-PCR) and the amplicons were sequenced. Finally, the data were statistically evaluated. Results: Quantitative RT-PCR revealed significant down-regulation of miR-374 (0.63-fold) and upregulation of APC (1.12-fold) and GSK-3β (1.22-fold) in CRC patients after five weeks of radiotherapy. Sequencing of PCR-produced amplicons confirmed the conservation of mature and precursor sequences encoding miR-374. miR-374 expression changed with time after radiotherapy treatment and related tumor grading. Increased age and tumor grade positively correlated with decreased miR-374 expression. Conclusions: miR-374 expression, and that of its two target genes, APC and GSK-3β, changed after radiotherapy. These genes can likely be used as diagnostic radiotherapy markers in CRC. SP - 614 EP - 621 AU - Ahmadi, Azam AU - Bayatiani, Mohamd Reza AU - Seif, Fatemeh AU - Ansari, Jamshid AU - Rashidi, Parisa AU - Moghadasi, Mona AU - Etemadi, Mobarakeh AD - Department of Medical Physics and Radiotherapy, Arak University of Medical Sciences and Khansari Hospital, Arak, Iran. KW - Biomarker KW - Colorectal cancer KW - Mir-374 KW - Radiotherapy. UR - http://rbmb.net/article-1-643-en.html DO - 10.52547/rbmb.10.4.614 ER -