%0 Journal Article %A Bakhtiyari, Nasim %A Sharifi, Akbar %A Aftabi, Younes %A Gilani, Neda %A Zafari, Venus %A Zafari, Venus %A Ansarin, Atefeh %A Seyedrezazadeh, Ensiyeh %T Association Between NOX4 And Nrf2 Genes in Non-Small-Cell Lung Carcinoma: A Case-Control Study %J Reports of Biochemistry and Molecular Biology %V 10 %N 2 %U http://rbmb.net/article-1-705-en.html %R 10.52547/rbmb.10.2.327 %D 2021 %K Gene expression, Non-small-cell lung cancer, NOX4, Nrf2., %X Background: Epithelial malignancy in lung cancer, which is initiated with myofibroblast differentiation and remodeling, promotes hypoxia and intracellular ROS generation most affected by the prototypical enzyme, NADPH oxidase 4 (NOX4). In addition, nuclear factor erythroid 2-related factor 2 (Nrf2) acts as a critical transcription factor by stimulating antioxidant proteins as redox homeostasis regulators. The aim of this study was to investigate a possible correlation between lung tissue NOX4 and Nrf2 genes (NOX4 and Nrf2) mRNA expression and bronchoalveolar lavage fluid (BALF) protein expression in non-small-cell lung carcinoma (NSCLC) patients. Methods: Samples from 25 patients with various NSCLC types and stages and 20 healthy controls were collected. NOX4 and Nrf2 mRNA were measured by qRT-PCR, and protein by western blot analysis. Results: NOX4 mRNA and protein expression was significantly up-regulated in NSCLC patients’ lung tissues and BALFs (p= 0.03 and 0.01, respectively). In addition, by adjusting for age, sex, and NSCLC types and stages, a significant and positive correlation was observed between NOX4 and Nrf2 mRNA expression (r= 0.927, p= 0.001). This was also true when not adjusted as above (r= 0.944, p< 0.001). Conclusions: NOX4 mRNA and protein expression is significantly up-regulated in NSCLC patients’ lung tissues and BALFs, and NOX4 and Nrf2 mRNA expression is positively correlated in NSCLC tissues. %> http://rbmb.net/article-1-705-en.pdf %P 327-333 %& 327 %! NOX4 and Nrf2 Correlation in NSCLC %9 Original Article %L A-10-770-1 %+ Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. %G eng %@ 2322-3480 %[ 2021