TY - JOUR T1 - HOTAIR and THRIL Long Non Coding RNAs and Their Target Genes in Rheumatoid Arthritis patients TT - JF - rbmb JO - rbmb VL - 10 IS - 4 UR - http://rbmb.net/article-1-785-en.html Y1 - 2022 SP - 697 EP - 710 KW - HOTAIR KW - Matrix metalloproteinase 2 and tumor necrosis factor alpha KW - Rheumatoid arthritis KW - THRIL. N2 - Background: Rheumatoid arthtritis (RA) is a chronic systemic inflammatory autoimmune disease characterized by irreversible joint damage and deformity. The aim of this study is to investigate THRIL and HOTAIR serum expression and their target genes in Egyptian RA patients and to evaluate their relationship to the clinico-pathological data. Methods: The present study included fifty-two RA patients and fifty-six healthy controls. RA patients were classified according to DAS28 score. All subjects were subjected to full history taking and clinical examination. Quantitative real time PCR was done to estimate the expression levels of serum THRIL and HOTAIR as well as their target genes tumor necrosis factor alpha (TNF-α) and metalloproteinase 2 (MMP- 2) were estimated by ELISA techniques. Results: Results revealed that both THRIL and HOTAIR were statistically over expressed in RA patients compared to healthy group with p-value< 0.05. Results showed as well that the target genes for those longnon coding RNAs, TNF-α and MMP-2, were also significantly higher in RA patients compared to healthy controls. Conclusions: Both THRIL and HOTAIR associated with their target genes, can be considered as diagnostic markers for RA. M3 10.52547/rbmb.10.4.697 ER -