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en Comparing the Biological Properties of Double-Stranded DNA Extracted from Human and Porcine Placenta and Salmon Sperm زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;">Background: Double-stranded fragmented extracellular DNA is a participant, inducer, and indicator of various processes occurring in the organism. When investigating the properties of extracellular DNA, the question regarding the specificity of exposure to DNA from different sources has always been raised. The aim of this study was to perform comparative assessment of biological properties of double-stranded DNA obtained from the human placenta, porcine placenta and salmon sperm. Methods: The intensity of leukocyte-stimulating effect of different dsDNA was assessed in mice after cyclophosphamide-induced cytoreduction. The stimulatory effect of different dsDNA on maturation and functions of human dendritic cells and the intensity of cytokine production by human whole blood cells was analyzed ex vivo. The oxidation level of the dsDNA was also compared. Results: Human placental DNA exhibited the strongest leukocyte-stimulating effect. DNA extracted from human and porcine placenta exhibited similar stimulatory action on maturation of dendritic cells, allostimulatory capacity, and ability of dendritic cells to induce generation of cytotoxic CD8+CD107a+ T cells in the mixed leukocyte reaction. DNA extracted from salmon sperm stimulated the maturation of dendritic cells, while having no effect on their allostimulatory capacity. DNA extracted from human and porcine placenta was shown to exhibit a stimulatory effect on cytokine secretion by human whole blood cells. The observed differences between the DNA preparations can be caused by the total methylation level and are not related to differences in oxidation level of DNA molecules. Conclusions: Human placental DNA exhibited the maximum combination of all biological effects.</div> Cytokines, Cytotoxic T cells, Dendritic cells, Double-stranded DNA. 577 589 http://rbmb.net/browse.php?a_code=A-10-1098-1&slc_lang=en&sid=1 Anastasia Proskurina asproskurina@gmail.com 100319475328460016787 100319475328460016787 No Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia. Valeriy Nikolin 100319475328460016788 100319475328460016788 No Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia. Nelly Popova 100319475328460016789 100319475328460016789 No Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia. Nikolay Varaksin 100319475328460016790 100319475328460016790 No JSC “Vector-Best”, Koltsovo, Novosibirsk Region, 630559, Russia. Tatiana Ryabicheva 100319475328460016791 100319475328460016791 No JSC “Vector-Best”, Koltsovo, Novosibirsk Region, 630559, Russia. Elizaveta Ershova 100319475328460016792 100319475328460016792 No Research Centre for Medical Genetics, Moscow, 115522, Russia. Svetlana Kostyuk 100319475328460016793 100319475328460016793 No Research Centre for Medical Genetics, Moscow, 115522, Russia. Olga Leplina 100319475328460016794 100319475328460016794 No Research Institute of Fundamental and Clinical Immunology, Novosibirsk, 630099, Russia. Alexandr Ostanin 100319475328460016795 100319475328460016795 No Research Institute of Fundamental and Clinical Immunology, Novosibirsk, 630099, Russia. Elena Chernykh 100319475328460016796 100319475328460016796 No Research Institute of Fundamental and Clinical Immunology, Novosibirsk, 630099, Russia. Sergey Bogachev labmolbiol@mail.ru 100319475328460016797 100319475328460016797 Yes Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia.