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en The Emerging Role of Circular RNA Homeodomain Interacting Protein Kinase 3 and Circular RNA 0046367 through Wnt/Beta-Catenin Pathway on the Pathogenesis of Nonalcoholic Steatohepatitis in Egyptian Patients زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;">Background: Non-alcoholic fatty liver disease is a major problem worldwide that needs non-invasive biomarkers for early diagnosis and treatment response assessment. We aimed to assess the correlation between circRNA-HIPK3 and miRNA-29a expression and its role as miRNA-29a sponge, as well as the correlation between circRNA-0046367 and miRNA-34a expression and its role as miRNA-34a sponge and their effect on regulation of the Wnt/β catenin pathway, which may provide a new target for treatment of non-alcoholic steatohepatitis. </div> <div style="text-align: justify;">Methods: the research was performed on 110 participants: group (I): fifty-five healthy donors served as controls and group (II): fifty-five patients with fatty liver pattern on abdominal ultrasound. Lipid profile and liver functions were assessed. RT-PCR was performed to assess the RNAs: circRNA-HIPK3, circRNA-0046367, miRNA-29a, miRNA-34a and Wnt mRNA gene expression. ELISA was performed to determine β-catenin protein levels. Results: miRNA-34a and circRNA-HIPK3 expression were significantly greater, while miRNA-29a and circRNA-0046367 expression were significantly less, in patients than in controls. Wnt/β-catenin regulated by miRNA-29a and miRNA-34a showed a significant decrease that leads to its abnormal effect on lipid metabolism. Conclusions: our results imply that miRNA-29a can be investigated as a target for circRNA-HIPK3, while miRNA-34a can be investigated as a target for circRNA-0046367, and that circRNA-HIPK3 and circRNA-0046367 may have emerging roles that can affect the pathogenesis of nonalcoholic steatohepatitis through the Wnt/β-catenin pathway and thus be used as therapeutic targets for the disease.</div> Circrna-HIPK3, circRNA-0046367, miRNA-29a, miRNA-34a, Wnt/β-catenin NASH. 614 625 http://rbmb.net/browse.php?a_code=A-10-1148-1&slc_lang=en&sid=1 Marwa Abdelgwad 100319475328460016810 100319475328460016810 No Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Egypt. Reem Zakaria Reem.Z.Ebrahim@kasralainy.edu.eg. 100319475328460016811 100319475328460016811 Yes Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Egypt. Samar Marzouk 100319475328460016812 100319475328460016812 No Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Egypt. Dina Sabry 100319475328460016813 100319475328460016813 No Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Egypt. Rasha Ahmed 100319475328460016814 100319475328460016814 No Endemic Medicine Department, Faculty of Medicine, Cairo University, Egypt. Hedy Ayman Badary 100319475328460016815 100319475328460016815 No Endemic Medicine Department, Faculty of Medicine, Cairo University, Egypt. Mai Samir 100319475328460016816 100319475328460016816 No Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Egypt.