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en Sodium Hydrosulfide Modification of Mesenchymal Stem Cell-Exosomes Improves Liver Function in CCL4-Induced Hepatic Injury in Mice زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;">Background: Liver diseases and injuries are important medical problems worldwide. Acute liver failure (ALF) is a clinical syndrome characterized by severe functional impairment and widespread death of hepatocytes. Liver transplantation is the only treatment available so far. Exosomes are nanovesicles originating from intracellular organelles. They regulate the cellular and molecular mechanisms of their recipient cells and have promising potential for clinical application in acute and chronic liver injuries. This study compares the effect of Sodium hydrosulfide (NaHS) modified exosomes with non-modified exosomes in CCL4-induced acute liver injury to ascertain their role in ameliorating hepatic injury. Methods: Human Mesenchymal stem cells (MSCs) were treated with or without NaHS (1 μmol) and exosomes were isolated using an exosome isolation kit. Male mice (8-12 weeks old) were randomly divided into four groups (n=6): 1-control, 2-PBS, 3- MSC-Exo, and 4- H2S-Exo. Animals received 2.8 ml/kg body weight of CCL4 solution intraperitoneally, and 24 h later MSC-Exo (non-modified), H2S-Exo (NaHS-modified), or PBS, was injected in the tail vein. Moreover, 24 h after Exo administration, mice were sacrificed for tissue and blood collection. Results: Administration of both MSC-Exo and H2S-Exo reduced inflammatory cytokines (IL-6, TNF-α), total oxidant levels, liver aminotransferases, and cellular apoptosis. Conclusions: MSC-Exo and H2S-Exo had hepato-protective effects against CCL4-induced liver injury in mice. Modification of cell culture medium with NaHS as an H2S donor enhances the therapeutic effects of MSC exosomes.</div> Acute liver failure (ALF), CCL4-induced liver injury, Exosomes, Mesenchymal stem cells (MSCs), Sodium hydrosulfide (NaHS). 644 655 http://rbmb.net/browse.php?a_code=A-10-1171-1&slc_lang=en&sid=1 Maryam Jafar Sameri M.Jafarsameri@Abadanums.ac.ir 100319475328460018267 100319475328460018267 Yes Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran & Physiology department, Abadan University of Medical Sciences, Abadan, Iran. Rafie Belali 100319475328460018268 100319475328460018268 No Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Niloofar Neisi 100319475328460018269 100319475328460018269 No Infectious and Tropical Diseases Research Center, Health Research Institute, Department of Medical virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Reza Noei Razliqi * noei.r@ajums.ac.ir. 100319475328460018270 100319475328460018270 No Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Seyed Ali Mard 100319475328460018271 100319475328460018271 No Persian Gulf Physiology Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Feryal Savari 100319475328460018272 100319475328460018272 No Department of basic sciences, Shoushtar Faculty of Medical Sciences, Shoushtar, Iran. Seyyed Saeed Azandeh 100319475328460018273 100319475328460018273 No Department of Anatomical Sciences, School of Medicine, Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.