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'); Reports of Biochemistry and Molecular Biology rbmb.net Basic Sciences http://rbmb.net 1 admin 2322-3480 2322-3480 10.61882/rbmb en jalali 1402 1 1 gregorian 2023 4 1 12 1 online 1 fulltext
en Role of Cannabinoid Type 2 Receptor Activation in Renal Fibrosis Induced by Unilateral Ureteric Obstruction in Rats زیست شناسی سلولی Cell Biology مقالات اصلی Original Article <div style="text-align: justify;"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt">Background:</span></span></span></i></b><b><i> </i></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt">Chronic kidney disease (CKD) ends mostly with renal fibrosis. The effect of CB2 receptor on renal fibrosis has been unclear. The aim of this study was to investigate the effect of CB2 receptor on renal fibrosis and the mechanisms behind it.</span></span></span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt">Methods:</span></span></span></i></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">50 adult male Sprague-Dawley rats were divided into 5 groups; normal, sham; rats had their ureters only manipulated, UUO; rats had their left ureters ligated, and JWH post; rats had their left ureters ligated and they received JWH 133 for 14 days, JWH pre+post; rats received JWH 133 for 14 days before and after UUO procedure. Serum creatinine and BUN were assessed together with tissue MDA, GSH, and catalase. Histopathological evaluation of the renal tissue by H&E and Masson&rsquo;s trichrome was done. Immunohistochemical staining for TGF-&beta;1, AQP1, Caspase-3, LC3B and p62 was performed. AQP1 and CB2 receptors genes expression was detected by quantitative RT-PCR.</span></span></span></span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="text-autospace:none"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt">Results:</span></span></span></i></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">UUO had caused severe damage in the renal tissue with reduction of the renal function parameter accompanied by increase in the collagen deposition with increase TGF-&beta;1 and decrease AQP1 expression.</span></span></span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="text-autospace:none"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt">Conclusions:</span></span></span></i></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt">The improvement of these parameters with JWH-133 suggests an anti-fibrotic role of CB2 receptor activation through reduction of oxidative stress, apoptosis, and autophagy.</span></span></span></span></span></span></span></span></div> AQP1, CB2 receptor, JWH-133, Renal fibrosis, UUO. 59 73 http://rbmb.net/browse.php?a_code=A-10-1194-1&slc_lang=en&sid=1 Mahmoud El Tohamy dr.m.eltohamy@mans.edu.eg 100319475328460017811 100319475328460017811 Yes Department of Medical Physiology, Faculty of Medicine, Mansoura University, Egypt. Mohamed Adel 100319475328460017812 100319475328460017812 No Department of Medical Physiology, Faculty of Medicine, Mansoura University, Egypt. Fayza Rashad El-Menabawy 100319475328460017813 100319475328460017813 No Department of Medical Physiology, Faculty of Medicine, Mansoura University, Egypt. Gad El Mawla Gad 100319475328460017814 100319475328460017814 No Department of Medical Physiology, Faculty of Medicine, Mansoura University, Egypt. Randa El-Gamal 100319475328460017815 100319475328460017815 No Department of Biochemistry, Faculty of Medicine, Mansoura University, Egypt. Hanaa El Serougy 100319475328460017816 100319475328460017816 No Department of Medical Physiology, Faculty of Medicine, Mansoura University, Egypt.