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en Genetic Regulation of Interleukin-6 and Interleukin-10 in COVID-19 Infection زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;">Background: The role and regulation mechanisms of the interleukin-6 and 10 (IL6 and IL-10) serum levels and the interaction between CD4+ and CD8+ lymphocytes with SARS-COV-2 IgM and IgG in the context of COVID-19 infection are not fully understood. Methods: This study was conducted on 45 COVID-19 patients and 45 healthy individuals. The IL-6 and IL-10 promoter methylation, IL-6 and IL-10 gene expression, SARS-COV-2 IgM, and IgG antibodies and CD4+ and CD8+ lymphocytes were studied by qMSP-PCR, Real-time PCR, ELISA, and flow cytometry techniques, respectively. Results: The male ratio and mean age of critically ill patients’ group were significantly higher in compared to controls (P< 0.05). IL-6 gene expression and serum levels were significantly increased in patients compared to controls (P=0.002, 0.001), but IL-6 promoter methylation was not significantly decreased in patients (P=0.835). The IL-10 promoter methylation and expression were not different between cases and controls (0.326, 0.455), but serum IL-10 levels were higher in patients (P< 0.001). The CD4+ and CD8+ lymphocytes decreased (P< 0.001) and mean SARS-COV-2 IgG increased (P=0.002) in the patients compared to controls. Conclusions: The COVID-19 disease result in severe complications in men and elderly. The serum levels of interleukin-6 and 10 increases in COVID-19 infection, and the gene expression of these two interleukins underlying in this increase. The serum levels of IL-6, IL-10 and SARS-COV-2 IgG as well as CD4+ and CD8+ lymphocyte counts should be investigated to monitor patients and predict the course of disease.</div> COVID-19, Gene Expression, Interleukin-6 (IL-6), Interleukin-10 (IL-10), SARS-COV-2, Promoter Methylation. 284 293 http://rbmb.net/browse.php?a_code=A-10-1031-2&slc_lang=en&sid=1 Zahra Rostami-Far 100319475328460017953 100319475328460017953 No Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan university of medical science, Sanandaj, Iran. Khaled Rahmani 100319475328460017954 100319475328460017954 No Liver and Digestive Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran. Kamran Mansouri 100319475328460017955 100319475328460017955 No Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran. Mohammad Bagher Khadem Erfan 100319475328460017956 100319475328460017956 No Department of Molecular Medicine, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran. Farhad Shaveisi-Zadeh 100319475328460017957 100319475328460017957 No Clinical Research Development Center, Imam Reza Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran. Bahram Nikkhoo 100319475328460017958 100319475328460017958 Yes Department of Pathology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.