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en Aberrant Methylation of the SOD1 GENE, its Expression and Enzyme Activity in the Placenta of Patients with Preeclampsia زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;">Background: Oxidative stress is involved in the pathogenesis of preeclampsia (PE). Dysregulation of SOD1 may be involved in the pathogenesis of PE. We examined and compared the methylation level of the promoter region (PMR) of the SOD1, gene expression, and enzyme activity of superoxide dismutase (SOD) in both placenta and maternal blood in PE women. Methods: A total of 140 blood samples and 40 placental tissue samples from PE and healthy pregnant controls were studied. The PMR of the SOD1 (Methylight PCR method), the expression (Real-time PCR), and its enzyme activity were investigated and compared in two groups. Results: The PMR of the SOD1 gene in the placental tissue of the patients was significantly increased compared to the control group (P= 0.008); this result was accompanied by a decrease in the expression of the gene and a decrease in the activity of the SOD enzyme. Meanwhile, the PMR of the SOD1 gene did not significantly change in the blood samples of the patients (P= 0.95), while a significant decrease in the expression of SOD1 (without a significant change in the SOD activity) was observed. Conclusions: The results showed significant changes in the PMR of the SOD1 gene and gene expression in placenta tissue. The results highlight the role of the placenta in complications during pregnancy and also revealed epigenetics as an important regulatory pathway in the pathogenesis of preeclampsia.</div> Preeclampsia, SOD1, Methylation, Gene expression, Oxidative stress. 112 119 http://rbmb.net/browse.php?a_code=A-10-1214-1&slc_lang=en&sid=1 Saba Zakeri 100319475328460017842 100319475328460017842 No Department of Clinical Biochemistry, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran. Zohreh Rahimi zrahimi@kums.ac.ir 100319475328460017843 100319475328460017843 Yes Department of Clinical Biochemistry, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran & Medical Biology Research Center, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran. And Department of Clinical Biochemistry, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran. Nazanin Jalilian 100319475328460017844 100319475328460017844 No Department of Clinical Biochemistry, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran. Asad Vaisi-Raygani 100319475328460017845 100319475328460017845 No Department of Clinical Biochemistry, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran. Ali Rezvani 100319475328460017846 100319475328460017846 No Department of Clinical Biochemistry, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran. Zahra Dastafkan 100319475328460017847 100319475328460017847 No Medical Genetics Laboratory, Kermanshah University of Medical Sciences, Kermanshah, Iran.