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en Possible Role of Oxidative Stress and Nrf2/HO-1 Pathway in Pentylenetetrazole-induced Epilepsy in Aged Rats زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;">Background: To examine the impact of aging on the response of rats to pentylenetetrazole (PTZ)-induction of epilepsy and the possible role of oxidative stress and nuclear factor erythroid 2-related factor 2 (Nrf2)/ heme oxygenase (HO)-1 pathway in this response. Methods: Forty male albino rats were equally allocated into 4 groups; 1) Young control (YC) group, aged 8-12 weeks, 2) Old control (OC) group, aged 24 months, 3) PTZ-Young group: young rats received PTZ (50 mg/Kg, i.p. every other day) for 2 weeks and 4) PTZ-Old group: as group 3 but rats were old. The seizure score stage and latency to the first jerk were recorded in rats. Redox state markers in brain tissues including malondialdehyde (MDA), catalase and total antioxidant capacity (TAC) were evaluated. Also, the expression of Nrf2 and HO-1 genes were measured in the brain tissues. Results: Old rats showed an early and a significant rise in the seizure score with PTZ administration and a significant drop in the seizure latency compared to young rats (P <0.01). Also, old rats showed a significantly higher MDA concentration and a significantly lower TAC and catalase activity than young rats (P <0.01). Moreover, the expression of Nrf2 and HO-1 was significantly lowered in old rats compared to young rats with PTZ administration (P < 0.01). Conclusions: Aging increases the vulnerability of rats to PTZ-induced epilepsy. An effect might come down to the up-regulation of oxidative stress and the down regulation of antioxidant pathways including Nrf2 and HO-1.</div> Aging, Epilepsy, HO-1, Nrf2, Oxidative stress, Pentylenetetrazole (PTZ). 147 158 http://rbmb.net/browse.php?a_code=A-10-1130-2&slc_lang=en&sid=1 Walaa Obydah menhag@mans.edu.eg 100319475328460017867 100319475328460017867 Yes Department of Medical Physiology, Faculty of Medicine, Mansoura University, Mansoura (35516), Egypt. Ahmed Fathi Abouelnaga 100319475328460017868 100319475328460017868 No Department of Animal Husbandry and Development of animal wealth, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt. Marwa Abass 100319475328460017869 100319475328460017869 No Department of Surgery, Anesthesiology, and Radiology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt. Somaya Saad 100319475328460017870 100319475328460017870 No Department of Medical Physiology, Faculty of Medicine, Mansoura University, Mansoura (35516), Egypt. Asmaa Yehia 100319475328460017871 100319475328460017871 No Department of Medical Physiology, Faculty of Medicine, Mansoura University, Mansoura (35516), Egypt. Omar Abd-Alhakem Ammar 100319475328460017872 100319475328460017872 No Basic Science Department, Delta University for Science and Technology, Gamasa, Egypt. Alaa Mohamed Badawy 100319475328460017873 100319475328460017873 No Department of Anatomy and Embryology, Faculty of Medicine, Mansoura University, Mansoura, 35516, Egypt. Mohie Mahmoud Ibrahim 100319475328460017874 100319475328460017874 No Department of Anatomy and Embryology, Faculty of Medicine, Mansoura University, Mansoura, 35516, Egypt. Abdelaziz Mohamed Hussein 100319475328460017875 100319475328460017875 No Department of Medical Physiology, Faculty of Medicine, Mansoura University, Mansoura (35516), Egypt.