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en Investigation of the Effect of Seminal Plasma Exosomes from the Normal and Oligoasthenoteratospermic Males in the Implantation Process زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;">Background: Seminal plasma exosomes are now recognized to play a complex role in the regulation of the female reproductive system infertility. The objective of this study was to assess the effect of exosomes derived from the sperm of men with oligoasthenoteratozoospermia on endometrial implantation-related genes. Methods: To isolate the exosomes, we employed an ultracentrifugation method on samples derived from 10 fertile men with normal sperm parameters and 10 men with oligoasthenoteratozoospermia. The size distribution and ultrastructure of the exosomes were then characterized using transmission electron microscopy and dynamic light scattering. We detected an exosome marker using western blot analysis and confirmed the cytoplasmic localization of the exosomes by incubating them with DiI dye and visualizing them using fluorescence microscopy. After 6 hours of in vitro treatment of endometrial epithelial cells with 100 µg/ml seminal exosome, the endometrial receptivity genes were examined using qRT-PCR. To perform data analysis and quantification, we utilized Image J and Prism software. P< 0.05 were considered statistically significant. Results: After 6 hours of treatment, the mRNA levels of MUC1, LIF, G-CSF, CX3CL1, and VEGF were significantly downregulated in the endometrial epithelial cells treated with oligoasthenoteratozoospermia exosomes compared to the normal group. Although changes were observed in the mean mRNA levels of IL8 and TGF-ß genes in the oligoasthenoteratozoospermia group compared to the normal group, these differences did not reach statistical significance (p > 0.05). Conclusions: Oligoasthenoteratozoospermia exosomes have a distinct effect on endometrial receptivity compared to normal exosomes, leading to reduced expression of implantation-related genes.</div> Embryo implantation, Endometrium, Exosome, Semen, Infertility. 294 305 http://rbmb.net/browse.php?a_code=A-10-1307-1&slc_lang=en&sid=1 Hadis Gholipour 100319475328460017959 100319475328460017959 No Department of Anatomy, School of Medicine, Iran University of Medical Science, Tehran, Iran. Fatemeh Sadat Amjadi 100319475328460017960 100319475328460017960 No Shahid Akbar Abadi Clinical Research Development unit (SHACRDU), School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Zahra Zandieh 100319475328460017961 100319475328460017961 No Shahid Akbar Abadi Clinical Research Development unit (SHACRDU), School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Mehdi Mehdizadeh 100319475328460017962 100319475328460017962 No Department of Anatomy, School of Medicine, Iran University of Medical Science, Tehran, Iran & Reproductive Sciences and Technology Research Center, Department of Anatomy, Iran University of Medical Sciences, Tehran, Iran. Marziyeh Ajdary 100319475328460017963 100319475328460017963 No Endometriosis Research Center, Iran University of Medical Sciences, Tehran, Iran. Ali Akbar Delbandi 100319475328460017964 100319475328460017964 No Reproductive Sciences and Technology Research Center, Department of Anatomy, Iran University of Medical Sciences, Tehran, Iran & Immunology Research Center, Immunology and Infectious Disease Institute, Iran University of Medical Sciences, Tehran, Iran. Azadeh Akbari Sene 100319475328460017965 100319475328460017965 No Shahid Akbar Abadi Clinical Research Development unit (SHACRDU), School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Reza Aflatoonian* r.aflatoonian@gmail.com 100319475328460017966 100319475328460017966 No Department of Endocrinology and Female Infertility at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. Mehrdad Bakhtiyari bakhtiyari1111@yahoo.com 100319475328460017967 100319475328460017967 Yes Department of Anatomy, School of Medicine, Iran University of Medical Science, Tehran, Iran.