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'); Reports of Biochemistry and Molecular Biology rbmb.net Basic Sciences http://rbmb.net 1 admin 2322-3480 2322-3480 10.61882/rbmb en jalali 1402 11 1 gregorian 2024 2 1 12 4 online 1 fulltext
en Interleukin-10 Gene Polymorphisms Modulate the Risk of Infertility in Chlamydia trachomatis Positive Kurdish Women in Erbil Province ایمنی شناسی Immunology مقالات اصلی Original Article <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span lang="EN-GB" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Background:</span></span></span></i></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">There is evident inter-individual variability in women&#39;s responses to Chlamydial infections and reproductive tract problems. Women&#39;s genetic variations within the Interleukin-10 (IL-10) gene have been linked to variances in response to <i>Chlamydia trachomatis</i> infection. This study was aimed to demonstrate the profound association of IL-10 with infertility and demonstrate the role of IL-10 (-592 C/A rs1800872) and (-1082 A>G rs1800896) single nucleotide polymorphism&nbsp;(SNPs) gene in the susceptibility and severity of a <i>C. trachomatis</i> infection.</span></span></span></span></span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Method</span></span></span></i></b><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">: </span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">In this evaluation study, serum IL-10 concentration was measured in 134 women diagnosed with infertility and 50 healthy volunteers by enzyme-linked immunosorbent assay (ELISA). The tetra-amplification refractory mutation system-PCR (T-ARMS-PCR) analysis was performed to detect the genotyping of the rs1800872 and rs1800896 SNPs genes.</span></span></span></span></span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Result</span></span></span></i></b><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">:</span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> Both female groups were positive for anti-chlamydial IgM antibody, but the intensity of response differed between cases. At the same time, the incidence of genital <i>C. trachomatis</i> by PCR<b> </b>was 46.2% in infertile women. The serum concentration of IL10 was lower in infertile women than healthy participants and higher in infertile <i>C. trachomatis</i>-positive women compared to infertile <i>C. trachomatis</i>-negative in all groups except endometriosis (Endo) infertility.<b> </b>In rs1800872, the CA genotype and C allele are associated with an increased risk for infertility, except in polycystic ovarian syndrome (PCOS), which is an A allele. In the case of rs1800896, the AG genotype and G allele show a greater risk for infertility.</span></span></span></span></span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Conclusions</span></span></span></i></b><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">: </span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Our results confirmed that rs1800872 and rs1800896 gene polymorphisms were associated with an increased risk of <i>C. trachomatis </i>infection.</span></span></span></span></span></span></span></span></span></div> Chlamydia trachomatis, Female infertility, IL-10, SNP 550 565 http://rbmb.net/browse.php?a_code=A-10-1340-1&slc_lang=en&sid=1 Lawin Ahmed Omar Lawin.omar@su.edu.krd 100319475328460020218 100319475328460020218 Yes Department of Biology, College of Education, Salahaddin University-Erbil, Erbil City, Kurdistan Region, Iraq Suhaila Nafee Darogha Nafee Darogha 100319475328460020219 100319475328460020219 No Department of Biology, College of Education, Salahaddin University-Erbil, Erbil City, Kurdistan Region, Iraq