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en Resolvin D1 (Rvd1) Attenuates In Vitro LPS-Stimulated Inflammation Through Downregulation of miR-155, miR -146, miR -148 and Krupple Like Factor 5 ایمنی شناسی Immunology مقالات اصلی Original Article <div style="text-align: justify;">Background: Chronic inflammation is associated with many inflammatory diseases. Specialized pro-resolving mediators (SPMs) are well known for their crucial role in promoting the resolution phase of inflammation and restoring tissue homeostasis. Resolvin D1 (RvD1) is an endogenous omega-3-derived lipid mediator with pro-resolving activity. This study aimed to evaluate the effect of Resolvin D1 (RvD1) on some inflammatory miRNAs (mir-155-5p, miR146a-5p and miR148-3p) and Krüppel-like factors 5 (KLF5) in an LPS-stimulated THP-1 preclinical model of inflammation. Methods: PMA-differentiated THP-1 cells (macrophages) were pre-incubated with or without various concentrations of RvD1 (10, 50, or 100 nM) for 2 h prior to stimulation by 1 μg/ml LPS. Un-stimulated PMA-differentiated THP-1 cells were as the control group. Then, the expression levels of target genes were evaluated by real-time PCR. Results: Compared with untreated macrophages, stimulation with 1 µg/ml LPS increased mRNA expression levels of TNF-α, KLF5, miR-155-5p, miR-146-5p, and miR-148a-3p. When the cells were exposed to various concentrations (10, 50 and 100 nM) of RvD1 for 2 h prior to LPS stimulation, the TNF-α, KLF5, miR-155-5p, miR-146-5p, and miR-148a-3p mRNA expression levels were significantly downregulated in a dose-dependent manner, compared to the LPS group. Conclusion: The results demonstrate that RvD1 can attenuate inflammatory response in LPS-stimulated macrophages. Our data also showed that RvD1 may exert anti-inflammatory effects by inhibiting miR-155-5p, miR-146a-5p, and miR-148-3p.</div> Chronic inflammation, Krupple like factors (KLF), Micro-RNAs, Resolvin D1 (RvD1), Macrophages. 566 574 http://rbmb.net/browse.php?a_code=A-10-556-4&slc_lang=en&sid=1 Jabar Amin Mohedin 100319475328460020220 100319475328460020220 No Student Research Committee, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran & Department of Laboratory, Ministry of Health, Sulamania, KRG, Iraq. Alireza Rezaiemanesh 100319475328460020221 100319475328460020221 No Department of immunology, school of medicine, Kermanshah University of Medical Science, Kermanshah, Iran. Sohila Asadi 100319475328460020222 100319475328460020222 No Department of biochemistry, school of medicine, Kermanshah University of Medical Science, Kermanshah, Iran. Maryam Haddadi 100319475328460020223 100319475328460020223 No Student Research Committee, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran. Bahroz Abdul Ahmed 100319475328460020224 100319475328460020224 No Student Research Committee, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran. Ali Gorgin Karaji 100319475328460020225 100319475328460020225 No Department of immunology, school of medicine, Kermanshah University of Medical Science, Kermanshah, Iran. Farhad Salari f.salari@kums.ac.ir 100319475328460020226 100319475328460020226 Yes Department of immunology, school of medicine, Kermanshah University of Medical Science, Kermanshah, Iran.