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en Detection of Methylene Tetrahydrofolate Reductase (MTHFR C677T) Mutation among Acute Lymphoblastic Leukemia in Sudanese Patients زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;">Background: A genetic polymorphism that causes abnormal folate metabolism may lead to genomic instability and increase susceptibility to malignancies such as Acute Lymphoblastic leukemia (ALL). The purpose of this research is to identify methylene tetrahydrofolate reductase (MTHFR C677T) (NCBI ID: 4524) mutation in ALL patients. Methods: The study was a descriptive case-control hospital-based study with one hundred Sudanese participants divided equally into fifty (50) Sudanese ALL diagnosed patients as cases and fifty (50) Sudanese individuals as controls. The MTHFR C677T mutant allele was detected using conventional PCR, with the primer sequence of MTHFR C677T F-TGAAGGAAGGTGTCTGCGGGA R-AGGACGGTGCGGTGAGAGTG. The study was conducted from January to March 2023, and samples were collected from the Radiation and Isotops Center at Khartoum Hospital. Results: The investigation revealed that 12 of the 50 patients in the case group (24%) had the MTHFR C677T mutant allele, and the study also revealed that there is significant correlation with the control group. There is no significant relationship between socio-demographic variables and MTHFR mutation detection in ALL patients. Also, the sociodemographic variables predictors of MTHFR mutation among ALL patients adjusted for smoking habit revealed no significant relationship. Conclusion: According to the findings of this study, the mutant allele of the Methylene Tetra Hydro Folate Reductase C677T was detected and demonstrated varying degrees of significance. It was concluded that the MTHFR C677T gene mutation was associated with acute lymphoblastic leukemia in Sudanese patients.  </div> ALL, MTHFR C677T, MTHFR protein, Mutation. 458 464 http://rbmb.net/browse.php?a_code=A-10-1371-1&slc_lang=en&sid=1 Waad Almuatasem Mohieldeen 100319475328460018596 100319475328460018596 No Department of Hematology and Immunohematology, Faculty of Medical Laboratory, National University, Sudan. Albara Ahmed 100319475328460018597 100319475328460018597 No Department of Hematology, Medical Laboratory Program, Alfajr College for Sciences and Technology, Sudan. Yousif Mohammed Elmosaad 100319475328460018598 100319475328460018598 No Department of Public Health, College of Applied Medical Sciences, King Faisal University, Saudi Arabia. Rania Saad Suliman 100319475328460018599 100319475328460018599 No Department of Clinical Laboratory Sciences, Prince Sultan Military College for Health Sciences, Dhahran, Saudi Arabia. Abdulaziz Alfahed 100319475328460018600 100319475328460018600 No Department of Medical Laboratory, College of Applied Medical Science, Prince Sattam Bin Abdulaziz University, Alkharj 11942, Saudi Arabia. Ahmed Hjazi 100319475328460018601 100319475328460018601 No Department of Medical Laboratory, College of Applied Medical Science, Prince Sattam Bin Abdulaziz University, Alkharj 11942, Saudi Arabia. Humood Al Shmrany 100319475328460018602 100319475328460018602 No Department of Medical Laboratory, College of Applied Medical Science, Prince Sattam Bin Abdulaziz University, Alkharj 11942, Saudi Arabia. Nora Hakami 100319475328460018603 100319475328460018603 No Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia. Mohammed Ageeli Hakami 100319475328460018604 100319475328460018604 No Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Al- Quwayiyah, Shaqra University, Riyadh, Saudi Arabia. Alhomidi Almotiri 100319475328460018605 100319475328460018605 No Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Dawadmi, Shaqra University, Riyadh, Saudi Arabia. Hisham Ali Waggiallah h.waggiallah@psau.edu.sa. 100319475328460018606 100319475328460018606 Yes Department of Medical Laboratory, College of Applied Medical Science, Prince Sattam Bin Abdulaziz University, Alkharj 11942, Saudi Arabia.