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en Circulating Levels of HOTAIR- lncRNA Are Associated with Disease Progression and Clinical Parameters in Type 2 Diabetes Patients بیوشیمی Biochemistry مقالات اصلی Original Article <div style="text-align: justify;">Background: Recent studies have implicated dysregulated long non-coding RNA (lncRNA) levels in the pathogenesis of type 2 diabetes (T2D). This study aimed to assess the expression of circulating HOTAIR and uc.48+, examining their correlation with clinical and biochemical variables in T2D patients, pre-diabetic individuals, and healthy controls. Methods: Peripheral blood levels of lncRNAs were quantified using QRT-PCR in 65 T2D patients, 63 pre-diabetic individuals, and 63 healthy subjects. Pathway enrichment analysis was conducted to explore the functional enrichment of lncRNA-miRNA targets. Results: Analysis revealed a significantly elevated circulating level of HOTAIR in both T2D (P < 0.0001) and pre-diabetic patients (P = 0.04) compared to controls. ROC analysis demonstrated that, at a cutoff value of 9.1, with a sensitivity of 80% and specificity of 62%, HOTAIR could distinguish T2D patients from controls (AUC = 0.723, 95% CI 0.637-0.799, P < 0.0001). Spearman correlation analysis identified a significant positive correlation between HOTAIR expression, HbA1c, and insulin resistance (P < 0.005). MiRNA enrichment analysis indicated significant enrichment of diabetes-related pathways among HOTAIR's miRNA targets. Conversely, no significant difference in uc.48+ circulating levels between groups was observed, but a significant positive correlation emerged between uc.48+ and systolic blood pressure. Conclusions: This study provides evidence that elevated HOTAIR expression levels are associated with T2D progression, suggesting their potential as biomarkers for early diagnosis and prognosis. </div> Diabetes Mellitus, HOTAIR, Long Non-Coding RNA, MicroRNAs. 448 457 http://rbmb.net/browse.php?a_code=A-10-1373-1&slc_lang=en&sid=1 Nafiseh Niknam 100319475328460018589 100319475328460018589 No Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran. Shekoofeh.nikooei@gmail.com Nikooei 100319475328460018590 100319475328460018590 No Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran. Hassan Ghasemi 100319475328460018591 100319475328460018591 No Department of Clinical Biochemistry, Abadan School of Medical Sciences, Abadan, Iran. Seyed Sajjad Zadian 100319475328460018592 100319475328460018592 No Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Kamran Goudarzi 100319475328460018593 100319475328460018593 No Department of Laboratory Sciences, Faculty of Paramedicine, Yasuj University of Medical Sciences, Yasuj, Iran. Seyed Majid Ahmadi 100319475328460018594 100319475328460018594 No Internal Medicine Department of Yasuj Medical School, Yasuj, Iran. Behnam Alipoor behnam.alipour@yums.ac.ir 100319475328460018595 100319475328460018595 Yes Department of Laboratory Sciences, Faculty of Paramedicine, Yasuj University of Medical Sciences, Yasuj, Iran.