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en Correlation of MicroRNA-125b, Sirtuin, and Signal Transducer and Activator of Transcription 3 with Biochemical Parameters and Risk Factors in Atherosclerosis Patients بیوشیمی Biochemistry مقالات اصلی Original Article <div style="text-align: justify;">Background: Atherosclerosis (AS) is an inflammatory disease linked to vascular events, with dysregulation of microRNA (miR)-125b, contributing to cardiovascular disease pathogenesis. Moreover, there is evidence of the involvement of signal transducer and activator of transcription 3 (STAT3) and sirtuin 6 (SIRT6) in AS. This study aimed to survey the expression levels of miR-125b, STAT3, and SIRT6 in the peripheral blood mononuclear cells (PBMCs) of AS patients and controls, and to find their correlations with biochemical parameters and risk factors. Methods: This study included blood samples from 45 controls and 45 AS patients, with PBMCs isolated using Ficoll solution. Expression levels of miR-125b, STAT3, and SIRT6 were determined via quantitative Real Time-PCR. Results: The findings revealed a significant increase in miR-125b levels in patients compared to controls (P = 0.017). However, alterations in STAT3 and SIRT6 expression were not significant (P> 0.05). There was no substantial relationship between miR-125b and STAT3 (P = 0.522) or SIRT6 (P = 0.88). miR-125b showed a significant relationship with atherogenic indexes and creatinine (P<0.05), while the association of SIRT6 with HDL and creatinine was significant (P<0.05). STAT3 exhibited high diagnostic power for identifying individuals at risk of heart disease and hypertension (P<0.05). Conclusions: STAT3 can serve as a valuable biomarker for detecting AS and AS-related risk factors. miR-125b and SIRT6 may be associated with AS lipid metabolism. However, further studies with larger sample sizes are recommended to mechanistically elucidate the association of these genes. Keywords: Atherosclerosis, MicroRNA-125b, STAT3, SIRT6, Leukocyte.</div> Atherosclerosis, microRNA-125b, Signal transducer and activator of transcription 3, Sirtuin 6, Leukocyte 631 642 http://rbmb.net/browse.php?a_code=A-10-1426-1&slc_lang=en&sid=1 Atefeh Mirderikvand 100319475328460020250 100319475328460020250 No Department of Clinical Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran. Gholamreza Shahsavari 100319475328460020251 100319475328460020251 No Department of Clinical Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran. Alireza Moayyed Kazemi 100319475328460020252 100319475328460020252 No Department of Internal Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran. Fatemeh Ahmadpour ahmadpour.f@lums.ac.ir 100319475328460020253 100319475328460020253 Yes Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Lorestan University of Medical Sciences, Khorramabad, Iran. Banafsheh Yalameha* yalameha@tbzmed.ac.ir 100319475328460020254 100319475328460020254 No Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran & Department of Biochemistry and Clinical Laboratories, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.