die(' Site is under construction

Dear site users

Site is under construction.

The site will be ready in less than 24 hours.

We are sorry for the inconvenience.

www.yektaweb.com

'); Reports of Biochemistry and Molecular Biology rbmb.net Basic Sciences http://rbmb.net 1 admin 2322-3480 2322-3480 10.61882/rbmb en jalali 1396 7 1 gregorian 2017 10 1 6 1 online 1 fulltext
en Production and Evaluation of Specific Single-Chain Antibodies against CTLA-4 for Cancer-Targeted Therapy تخصصي Special مقالات اصلی Original Article <p style="text-align: justify;"><strong><em>Background:</em></strong> &nbsp;Cytotoxic T lymphocyte&ndash;associated antigen 4 (CTLA-4) molecules are expressed on T-cells and inhibit their function by inhibiting activation of subsequent T-cell molecular pathways. Blocking of CTLA-4 inhibits the growth of malignant tumor cells. Anti-CTLA-4 monoclonal antibodies activate the immune system against cancer. Due to several advantages of single-chain antibodies (scFvs) compared to monoclonal antibodies in cancer immunotherapy, specific anti-CTLA-4 scFvs (single-chain variable fragment) were selected in this study.</p> <p style="text-align: justify;"><strong><em>Methods:</em></strong> A phage antibody display library of scFvs was analyzed and a panning process was performed against an immunodominant epitope of CTLA-4. PCR and DNA fingerprinting were used to differentiate the specific clones. The specificity of the selected clones was investigated by phage ELISA (Enzyme-linked immunosorbent assay).</p> <p style="text-align: justify;"><strong><em>Results:</em></strong> Two specific clones with frequencies of 35 and 20% were identified. The clones reacted with the corresponding epitope on ELISA, while no reactivity was observed with an unrelated peptide, M13KO7 helper phage, unrelated scFvs, or no peptide as negative controls.</p> <p style="text-align: justify;"><strong><em>Conclusions:</em></strong> Targeted therapy against cancer markers is an ideal treatment strategy. Specific human anti-CTLA-4scFvs were selected in this study. These scFvs bound the related epitope. These antibodies have the potential to be used for targeted therapy, where the blocking of CTLA4 receptor is needed. The study suggests further evaluation of the selected scFvs to reveal the effects of the selected antibodies.</p> Cancer immunotherapy, CTLA-4, ScFv antibodies 8 14 http://rbmb.net/browse.php?a_code=A-10-1-78&slc_lang=en&sid=1 Farideh Hosseinzadeh 10031947532846002199 10031947532846002199 No Shiraz HIV/AIDS Research Center, Shiraz University of Medical Sciences, Shiraz, Iran-Recombinant antibody laboratory, Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran Saeed Mohammadi 10031947532846002200 10031947532846002200 No Shiraz HIV/AIDS Research Center, Shiraz University of Medical Sciences, Shiraz, Iran-Recombinant antibody laboratory, Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran Foroogh Nejatollahi nejatollaf@sums.ac.ir 10031947532846002201 10031947532846002201 Yes Shiraz HIV/AIDS Research Center, Shiraz University of Medical Sciences, Shiraz, Iran-Recombinant antibody laboratory, Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran