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'); Reports of Biochemistry and Molecular Biology rbmb.net Basic Sciences http://rbmb.net 1 admin 2322-3480 2322-3480 10.61882/rbmb en jalali 1403 1 1 gregorian 2024 4 1 13 1 online 1 fulltext
en Association of ABCB1(Rs10276036, C/T) Gene, IL-18, and TNFα as Risk Factors for Nephrotic Syndrome Incidence زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div class="WordSection1"> <div style="text-align: justify;"><span style="page:WordSection1"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">Background:</span></span></span></span></i></b><b><i> </i></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">The most common cause of Nephrotic Syndrome (NS) in children is idiopathic NS, also called nephrosis. The most prominent clinical signs are hyperlipidemia, severe proteinuria, edema, swelling of body tissues, and an increased risk of infection. The object of this study was to examine the correlation of the ABCB1 gene (rs10276036, C > T), IL-18, and TNF&alpha; to the prevalence of NS among Egyptian children having NS.</span></span></span></span></span></span></span></span><br> <br> <span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">Methods:</span></span></span></span></i></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt"> This study included 100 participants with NS and 100 healthy controls. To analyze the ABCB1 gene (rs10276036 C >T) variant PCR technique was used. IL-18 and TNF levels were estimated using Enzyme-Linked Immunosorbent Assay (ELISA).</span></span></span></span></span></span></span></span><br> <br> <span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">Results:</span></span></span></span></i></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt"> Increased frequency of CT and TT genotypes of the ABCB1 gene (rs10276036 C / T) in NS patients compared to controls, with p-value = 0.001, OR = 2.270, CI = (1.550-3.327) for CT genotype and p-value = 0.001, OR = 5.070, CI = (2.463-10.438) for TT genotype. The frequencies of ABCB1 (rs10276036 C >T) genotypes were statistically significant in the dominant model (OR 2.560;<i> p<</i> 0.001) and in the recessive model OR, 3.231; <i>p</i>= 0.001). Significantly high levels of both IL-18 and TNF&alpha; were found in NS patients compared to controls.</span></span></span></span></span></span></span></span><br> <br> <span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">C<i>onclusion:</i></span></span></span></span></b><i> </i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">The ABCB1gene (rs10276036 C/T), IL-18, and TNF&alpha; are associated with the prevalence of NS in Egyptian children and might be considered as independent risk factors for its incidence.</span></span></span></span></span></span></span></span><br> <span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></span></span></span></span></div> <div align="center" class="CxSpMiddle" style="text-align:center"> <hr align="center" noshade="noshade" size="2" style="color:#0f243e" width="100%" ></div> </div> <div style="text-align: justify;"><div aria-label="Page Break" class="cke_pagebreak" contenteditable="false" data-cke-display-name="pagebreak" data-cke-pagebreak="1" style="page-break-after:always" title="Page Break"></div></div> ATP Binding Cassette Transporter, Interleukin-18, Polymerase Chain Reaction, Subfamily B, Tumor Necrosis Factor-alpha. 67 78 http://rbmb.net/browse.php?a_code=A-10-1475-1&slc_lang=en&sid=1 Eglal Aly Hassan 100319475328460020304 100319475328460020304 No Chemistry Department, Faculty of Science, Mansoura University, Mansoura 35516, Egypt. Afaf Mohamed Elsaid 100319475328460020305 100319475328460020305 No Genetic Unit, Children Hospital, Mansoura University, Mansoura 35516, Egypt. Ahmed Mahmoud El-Refaey 100319475328460020306 100319475328460020306 No Department of Pediatrics, Mansoura University Children's Hospital, Mansoura University, Mansoura 35516, Egypt. Mohammed Abou Elzahab 100319475328460020307 100319475328460020307 No Chemistry Department, Faculty of Science, Mansoura University, Mansoura 35516, Egypt. Magdy Mahfouz Youssef 100319475328460020308 100319475328460020308 No Chemistry Department, Faculty of Science, Mansoura University, Mansoura 35516, Egypt. Rehab Elmougy relmougy@mans.edu.eg. 100319475328460020309 100319475328460020309 Yes Chemistry Department, Faculty of Science, Mansoura University, Mansoura 35516, Egypt.