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'); Reports of Biochemistry and Molecular Biology rbmb.net Basic Sciences http://rbmb.net 1 admin 2322-3480 2322-3480 10.61882/rbmb en jalali 1403 1 1 gregorian 2024 4 1 13 1 online 1 fulltext
en TH1/TH2 Cytokine Profile and Their Relationship with Hematological Parameters in Patients with Acute Limb Ischemia ایمنی شناسی Immunology مقالات اصلی Original Article <span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">Background:</span></span></span></span></i></b><b><i> </i></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">The progression of acute limb ischemia (ALI) is being significantly influenced by changes in immune system function. The study aimed to determine the dominant immune cell responses (Th1 or Th2) in ALI patients by measuring serum levels of IL-4, IL-12, and IFN-&gamma;.<b> </b>Previous studies indicate altered cytokine levels in cerebral ischemia, but there is no prior research on these cytokines in ALI patients.</span></span></span></span></span></span></span></span><br> <br> <span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">Methods:</span></span></span></span></i></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt"> This study involved 34 patients with ALI and 34 healthy controls. Blood samples were analyzed for hematological factors such as erythrocyte sedimentation rate (ESR), white blood cell (WBC) count, red blood cell (RBC) count, platelet (Plt) count, hemoglobin (Hb), and hematocrit (HCT). The levels of serum cytokines IL-4, IL-12, and IFN-&gamma; were measured in both patients and control subjects using enzyme-linked immunosorbent assay (ELISA). The statistical analyses were conducted using SPSS and GraphPad Prism.</span></span></span></span></span></span></span></span><br> <br> <span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">Results:</span></span></span></span></i></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt"> The results showed that serum levels of IL-4 in ALI patients did not significantly differ from those in control groups. Acute limb ischemia exhibited significantly elevated levels of IL-12 and IFN-&gamma; compared to healthy individuals. In addition, no correlation between the production of cytokines and the hematological parameters was found.</span></span></span></span></span></span></span></span><br> <br> <span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="tab-stops:396.55pt"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">Conclusion:</span></span></span></span></i></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt"> Th1 responses are believed to play a role in the pathogenesis of ALI, but further research is needed to fully understand their exact role.</span></span></span></span></span></span></span></span> Fibrinogen, ORF-9b protein, SARS-CoV-2, Serum Albumin. 31 39 http://rbmb.net/browse.php?a_code=A-10-225-1&slc_lang=en&sid=1 Jamal Jalili Shahri 100319475328460020281 100319475328460020281 No Vascular and Endovascular Surgery Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Mohammad Hadi Saeed Modaghegh 100319475328460020282 100319475328460020282 No Vascular and Endovascular Surgery Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Hamid Tanzadehpanah 100319475328460020283 100319475328460020283 No Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Motahare Ebrahimnejad 100319475328460020284 100319475328460020284 No Department of Pathology, Mashhad University of Medical Sciences, Mashhad, Iran. Hanie Mahaki Mahakih991@mums.ac.ir 100319475328460020285 100319475328460020285 Yes Vascular and Endovascular Surgery Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.