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en The Utile Efficacy of Zinc and Selenium on Induction of White Adipose Tissue Browning زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;">Background: We investigated the effects of zinc (Zn) and selenium (Se) (co)-supplementation (individually and in combination) on the browning process of white (WAT) to brown adipose tissue (BAT). Method: We randomly allocated obese male Wistar rats (n= 40) into five groups that were fed a high-fat diet (HFD). The control group received only HFD. The intervention groups were as follows: 1) Zn administered zinc sulfate; 2) Se administered sodium selenate; 3) Se+Zn administered combination; 4) CRD placed on a calorie restriction diet. After eight weeks of intervention, the expression of selected genes, UCP-1, PGC-1α, PPAR-α, PPAR-γ, PRDM16, and were assessed. Histological assessment of adipose and the liver tissues was also conducted using the H&E stain method. Results: The findings revealed that, expression of UCP-1 was significantly increased in the Zn , Se , and Se+Zn groups in both WAT and BAT. It was highest in the HFD + Zn + Se (P< 0.001, P= 0.029) group. In addition, there was a decrease in UCP-1 gene expression in the CRD group, however, it was not statistically significant. Further, the expression of PPAR-γ and ZAG gene was significantly enhanced in the HFD + Zn (P< 0.001, P= 0.029) and HFD + Zn + Se (P< 0.001, P= 0.007) groups, rather no significant difference was found in the HFD + Se (P> 0.05) group. Furthermore, morphological changes in tissues were consistent with the outcomes of molecular experiments. Conclusion: The findings from this study suggest that Zn and Se supplementation, particularly when combined, may effectively enhance WAT browning and thermogenesis. This could provide a promising avenue for addressing obesity through novel therapeutic strategies.</div> Adipocytes, Adipose Tissue, Browning, Obesity, Thermogenesis. 258 270 http://rbmb.net/browse.php?a_code=A-10-1523-1&slc_lang=en&sid=1 Motahareh Hasani dr.hasani@goums.ac.ir 100319475328460022579 100319475328460022579 Yes Department of Nutrition, School of Health, Golestan University of Medical Sciences, Gorgan, Iran. Elham Ahmadi 100319475328460022580 100319475328460022580 No School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Vahid Monfared vahidmonfared52@gmail.com 100319475328460022581 100319475328460022581 No Skeletal Biology Laboratory, College of Health, Oregon State University, Corvallis, OR 97331, USA. Maryam Khazdouz 100319475328460022582 100319475328460022582 No Growth and Development Research Center, Children’s Medical Center, Tehran University of Medical Sciences, Tehran, Iran. Javad Heshmati 100319475328460022583 100319475328460022583 No Department of Public Health, Songhor Healthcare Center, Kermanshah University of Medical Sciences, Kermanshah, Iran. Farhad Vahid 100319475328460022584 100319475328460022584 No Nutrition and Health Research Group, Department of Precision Health, Luxembourg Institute of Health, Luxembourg. Mohammadreza Vafa 100319475328460022585 100319475328460022585 No Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran. Ali Jafari 100319475328460022586 100319475328460022586 No Student Research Committee, Department of Community Nutrition, Faculty of Nutrition Sciences and Food Technology, National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran.