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'); Reports of Biochemistry and Molecular Biology rbmb.net Basic Sciences http://rbmb.net 1 admin 2322-3480 2322-3480 10.61882/rbmb en jalali 1403 8 1 gregorian 2024 11 1 13 3 online 1 fulltext
en Effect of Active Compounds of Eruca Sativa Plant Extract on Staphylococcus aureus Bacteria بیوشیمی Biochemistry مقالات اصلی Original Article <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">Background:</span></span></span></span></i></b><b><i> </i></b><span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">Medicinal plants play an important role in agricultural production due to their therapeutic significance, particularly in the treatment of various pathological conditions.</span></span></span></span></span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">Methods:</span></span></span></span></i></b><span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt"> Active compounds in Eruca sativa were identified using High-Performance Liquid Chromatography (HPLC). Fifty isolates of <i>Staphylococcus aureus</i> (<i>S. aureus</i>) bacteria were obtained. The effect of Eruca sativa plant extract on biofilm formation of bacterial isolates was tested using the standard plate method.</span></span></span></span></span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">Results:</span></span></span></span></i></b><span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt"> The highest percentage of S. aureus was found in wound samples, with 22 isolates (44%). The isolates showed variability in their ability to form biofilms. The efficacy test revealed that plants treated with different concentrations of brassinolide (0, 1.5, 2.5, 3.5 mg/L) and Eruca sativa showed inhibition of S. aureus growth in isolates (S2, S7, S10, S15, S16, S31, S42, S48, S50) with alcohol concentrations (5, 25, 75 mg/ml). The effect of Eruca sativa plant extract was dependent on the concentration of brassinolide applied. The highest inhibition was observed with brassinolide concentration of 3.5 mg/L-1 and alcohol extract concentrations of 50 and 75 mg/ml.</span></span></span></span></span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">Conclusion:</span></span></span></span></i></b><span lang="EN-CA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt"> The alcoholic extract from the leaves of Eruca sativa, combined with brassinolide, proved effective in inhibiting the growth and biofilm formation of <i>S. aureus</i> bacteria.</span></span></span></span></span></span></span></span></span></div> Biofilm, Eruca Sativa, HPLC, Staphylococcus aureus. 394 404 http://rbmb.net/browse.php?a_code=A-10-1537-1&slc_lang=en&sid=1 Alaa Salim Sadah Al-Halfi aalaa.sada2102p@ihcoedu.uobaghdad.edu.iq. 100319475328460020761 100319475328460020761 Yes Department of Biology, College of Education for Pure Science/ Ibn Al-Haitham, University of Baghdad, Baghdad, Iraq. Maher Zaki Faisal Al-Shammari 100319475328460020762 100319475328460020762 No Department of Biology, College of Education for Pure Science/ Ibn Al-Haitham, University of Baghdad, Baghdad, Iraq. Suaad Khalil Ibrahim 100319475328460020763 100319475328460020763 No Department of Biology, College of Education for Pure Science/ Ibn Al-Haitham, University of Baghdad, Baghdad, Iraq. Sohad Abdulkaleg Alshareef 100319475328460020764 100319475328460020764 No University College of Duba, University of Tabuk, Tabuk, 71491, Saudi Arabia.