die(' Site is under construction

Dear site users

Site is under construction.

The site will be ready in less than 24 hours.

We are sorry for the inconvenience.

www.yektaweb.com

'); Reports of Biochemistry and Molecular Biology rbmb.net Basic Sciences http://rbmb.net 1 admin 2322-3480 2322-3480 10.61882/rbmb en jalali 1403 8 1 gregorian 2024 11 1 13 3 online 1 fulltext
en The Relationship Between the Growth of Fusobacterium nucleatum ATCC 25586 in Glucose-Enriched Media and Protein Activity through Fourier Transform Infrared (FTIR) زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">Background:</span></span></span></span></i></b><b><i> </i></b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">Fusobacterium nucleatum</span></span></span></span></i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt"> (<i>F. nucleatum</i>) is known to increase in number under hyperglycemic conditions, as it is thought to utilize glucose as a nutrient source. The process of glucose utilization in bacteria occurs with the assistance of enzymatic proteins such as glucokinase. This study aims to investigate the glucose utilization by <i>F. nucleatum</i> ATCC 25586 by examining its growth in glucose-enriched media and its relationship with protein activity through FTIR analysis.</span></span></span></span></span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">Methods</span></span></span></span></i></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">: <i>F. nucleatum</i> ATCC 25586 was cultured in media enriched with 2%, 1%, 0.75%, 0.5%, and 0.25% glucose. Its growth was measured using a spectrophotometer, and protein activity was assessed with FTIR at 24 and 48 hours of incubation.</span></span></span></span></span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">Results:</span></span></span></span></i></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt"> The results showed that <i>F. nucleatum </i>could utilize glucose as a nutrient source, indicated by growth and protein activity. The maximum growth of <i>F. nucleatum</i> occurred at a 0.75% glucose concentration at 24 hours. However, the Kruskal-Wallis test showed no significant differences in the growth and protein activity of <i>F. nucleatum</i> across the five glucose concentrations (growth, <i>p</i>=0.271 and protein, <i>p</i>=0.149). Spearman correlation analysis indicated no correlation between the growth and protein activity of <i>F. nucleatum</i> (<i>p</i>=0.323). The protein activity of <i>F. nucleatum</i> remained stable across various growth levels.</span></span></span></span></span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="tab-stops:396.55pt"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">Conclusion</span></span></span></span></i></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">: It can be concluded that glucose could influence the growth of <i>F. nucleatum</i>, although the growth and protein activity of the bacteria did not differ significantly based on glucose concentration. <i>F. nucleatum </i>grown in various glucose concentrations exhibits stable protein activity.</span></span></span></span></span></span></span></span></span></div> ATCC 25586, F. nucleatum, Glucose-Enriched Media, Hyperglycemic. 310 321 http://rbmb.net/browse.php?a_code=A-10-1592-1&slc_lang=en&sid=1 Dewi Saputri dewisaputri@usk.ac.id. 100319475328460020710 100319475328460020710 Yes Department of Periodontics, Faculty of Dentistry, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia. Zulfan Muhammad Alibasyah 100319475328460020711 100319475328460020711 No Department of Periodontics, Faculty of Dentistry, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia. Sunnati Nuzulul Ismi 100319475328460020712 100319475328460020712 No Department of Periodontics, Faculty of Dentistry, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia. Ardelia Arfirosa 100319475328460020713 100319475328460020713 No Department of Periodontics, Faculty of Dentistry, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia.