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en hsa_circ_0004121 and hsa_circ_0030162 Differentially Expressed in Plasma of Patients with Recurrent Implantation Failure زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;">Background: Recurrent or repeated implantation failure (RIF) is a significant challenge that hampers the success rate of assisted reproductive technology (ART) in achieving pregnancy. The underlying mechanisms of RIF remain unclear. Recent studies have identified distinct expression patterns of circular RNAs (circRNAs) in the endometrial tissues of individuals with RIF. The objective of this research is to evaluate the expression of six candidate circRNAs in the plasma of RIF patients. Methods: The study included a total of sixty participants, comprising 30 RIF patients and 30 age-matched controls. Specific primers were designed for six circRNAs (hsa_circ_0001713, hsa_circ_0004121, hsa_circ_0030162, hsa_circ_0034642, hsa_circ_0034762, and hsa_circ_0092337), and expression analysis was carried out by RT-qPCR. Blood samples were collected from individuals during the implantation window, and cell-free RNA was extracted from plasma. Statistical analysis was performed using Graphpad Prism software. Results: Control and RIF groups had mean ages of 34.68 ± 6.2 and 36.80 ± 3.8 years, respectively. Plasma from RIF patients showed significant downregulation of hsa_circ_0030162 (p=0.02) and upregulation of hsa_circ_0004121 (p=0.003) compared to controls. Bioinformatic analysis predicted hsa-miR-125a-3p and hsa-miR-125a-5p as potential targets of hsa_circ_0030162 and hsa_circ_0004121, respectively. Conclusion: This study demonstrates the differential plasma expression of hsa_circ_0030162 and hsa_circ_0004121 in RIF patients, consistent their expression in endometrial tissue. These circRNAs may contribute to RIF pathogenesis. Further research is needed to validate these findings and explore their clinical utility.</div> Circular RNA, Plasma, Pregnancy, Recurrent implantation failure. 428 437 http://rbmb.net/browse.php?a_code=A-10-1669-1&slc_lang=en&sid=1 Majid Zaki-Dizaji 100319475328460020656 100319475328460020656 No Human Genetics Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. Mostafa Saeedinia 100319475328460020657 100319475328460020657 No Human Genetics Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. Parisa Derogar 100319475328460020658 100319475328460020658 No Human Genetics Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. Behnoosh Jamshidi 100319475328460020659 100319475328460020659 No Human Genetics Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. Masoumeh Masoumi 100319475328460020660 100319475328460020660 No Vali-e-Asr Reproductive Health Research Center, Family Health Research Institute, Tehran University of Medical Sciences, Tehran, Iran. Zohreh Heidary z.heidary2016@gmail.com 100319475328460020661 100319475328460020661 Yes Vali-e-Asr Reproductive Health Research Center, Family Health Research Institute, Tehran University of Medical Sciences, Tehran, Iran.