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en Influence of Vitamin D Receptor Gene Polymorphisms on Response to Pegylated Interferon in Chronic Hepatitis B Egyptian Patients زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article Background: We explored the effect of vitamin D receptor gene (VDR) polymorphisms in response to PEG-IFN treatment in Egyptian chronic hepatitis B (CHB) patients. Methods: Two hundred hepatitis B virus (HBV) patients (42.3±10.7 years) on PEG-IFN α-2a (180 μg /kg for 48 weeks) and one hundred control subjects (37.3 ±12 years) were enrolled in the study. Vitamin D levels and hepatitis B surface antigen (HBsAg) expression were assessed by ELISA. VDR polymorphisms FokI T>C (rs 10735810), BsmI A>G (rs 1544410), ApaI (rs7975253), and TaqI C>T (rs 731236), were genotyped using real-time PCR. Results: Hepatitis B virus patients expressed significantly greater AST (p=< 0.00001) and ALT (P=< 0.00001), and significantly less vitamin D (P=0.01), than control subjects. Patients with Ff or ff alleles of the FokI single-nucleotide polymorphism (SNP), bb alleles of BsmI SNP, or TT alleles of the Taq1 single nucleotide polymorphisms (SNP) showed greater response to PEG-IFN therapy than those with the FF (P=0.02 and P=0.0002), Bb (P=0.023), or Tt/tt alleles (P=0.01 and P=0.004 respectively). Logistic stepwise regression showed that HBV DNA (r: 0.910, P< .00001), FokI SNP polymorphism (r: 0.919, (P=0.037) and bAt haplotype (r: .926, (P=0.043) are independent factors that determine PEG-IFN treatment response in the HBV-infected patients. Conclusions: VDR gene polymorphisms may be used as treatment response predictors in HBV patients receiving PEG-IFN. FokI SNP and bAt haplotype are independent factors that that can be used to determine PEG-IFN treatment responses in HBV-infected patients. Egypt, Hepatitis B virus, PEGylated interferon, Vitamin D receptor polymorphism. 186 196 http://rbmb.net/browse.php?a_code=A-10-78-1&slc_lang=en&sid=1 Gomaa Mostafa-Hedeab gomaa_hedeab@yahoo.com 10031947532846006304 10031947532846006304 No Pharmacology department, Faculty of Medicine, Beni Suef University – Egypt & Medical College, Al-Jouf University, Al-Jawf, Saudi Arabia Dina Sabry dinasabry@kasralainy.edu.eg 10031947532846006305 10031947532846006305 Yes Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Cairo, Egypt. Ghada Mostafa Abdelaziz dinnasabry@yahoo.com 10031947532846006306 10031947532846006306 No Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Beni Suef University, Beni Suef, Egypt. Manal Ewaiss manal_ewaiss@yahoo.com 10031947532846006307 10031947532846006307 No Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Beni Suef University, Beni Suef, Egypt & Medical College, Al-Jouf University, Al-Jawf, Saudi Arabia Nagla Adli naglaadli@yahoo.com 10031947532846006308 10031947532846006308 No Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Beni Suef University, Beni Suef, Egypt. Wael Fathy wael_fathy74@yahoo.com 10031947532846006309 10031947532846006309 No Tropical medicine Department, Faculty of Medicine, Beni Seuf University, Beni Suef, Egypt.