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en Mesenchymal Stem Cells Differentiate to Endothelial Cells Using Recombinant Vascular Endothelial Growth Factor –A زیست شناسی سلولی Cell Biology مقالات اصلی Original Article Background: Vascular endothelial growth factor-A (VEGF-A), an endothelial cell-specific mitogen produced by various cell types, plays important roles in cell differentiation and proliferation. In this study we investigated the effect of recombinant VEGF-A on differentiation of mesenchymal stem cells (MSCs) to endothelial cells (ECs). Methods: VEGF-A was expressed in E. coli BL21 (DE3) and BL21 pLysS competent cells with the pET32a expression vector. Recombinant VEGF-A protein expression was verified by SDS-PAGE and western blotting. Mesenchymal stem cell differentiation to ECs in the presence of VEGF-A was evaluated by flow cytometry and fluorescence microscopy. Results: Recombinant VEGF-A was produced in E. coli BL21 (DE3) cells at 0.8 mg/mL concentration. Expression of CD31 and CD 144 was significantly greater, while expression of CD90, CD73, and CD44 was significantly less, in MSCs treated with our recombinant VEGF-A than in those treated with the commercial protein (p < 0.05). Conclusions: Recombinant VEGF-A expressed in a prokaryotic system can induce MSCs differentiation to ECs and can be used in research and likely therapeutic applications. Cell differentiation, Endothelial cell, Mesenchymal stem cell, Vascular Endothelial Growth Factor A 144 150 http://rbmb.net/browse.php?a_code=A-10-74-1&slc_lang=en&sid=1 Mohsen Khaki mohsenkhaki1338@yahoo.com 10031947532846006275 10031947532846006275 No Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran-Department of Microbiology and Immunology, School of Medicine, Arak University of Medical Sciences, Arak, Iran. Ali Hatef Salmanian hatef44@yahoo.com 10031947532846006276 10031947532846006276 No National Institute for Genetic Engineering and Biotechnology, Tehran. Iran. Hamid Abtahi abtahi@arakmu.ac.ir 10031947532846006277 10031947532846006277 No Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran. Ali Ganji aliganjy_1360@yahoo.com 10031947532846006278 10031947532846006278 No Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran-Department of Microbiology and Immunology, School of Medicine, Arak University of Medical Sciences, Arak, Iran. Ghasem Mosayebi ghasemmosayebi@arakmu.ac.ir 10031947532846006279 10031947532846006279 Yes Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran-Department of Microbiology and Immunology, School of Medicine, Arak University of Medical Sciences, Arak, Iran.