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en Potential Effects of Selenium And N-Acetylcysteine Supplementation in Ameliorating Cardinal Symptoms of Nω-Nitro-L-Arginine Methyl Ester Hydrochloride (L-NAME) Induced Preeclampsia in Wistar Rats بیوشیمی Biochemistry مقالات اصلی Original Article <div style="text-align: justify;">Background: Preeclampsia (PE) is a hypertensive disorder in pregnancy affecting multiple organ systems. This study hypothesized that oxidative stress and inflammatory responses contribute to the pathogenesis of Preeclampsia, and that selenium and N-acetylcysteine (NAC) could mitigate these effects. Methods: The study was initiated after approval from the Institutional Animal Ethics Committee. Twenty-four female Wistar rats were divided equally into four groups. Group I served as controls, while Groups II, III, and IV received Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME) to induce hypertension from day 10 to 20 of gestation. Additionally, Group III received selenium (240 μg/kg/day) and Group IV received NAC (160 mg/kg). On day 20, Blood Pressure (BP) monitoring and urine protein estimation were carried out to assess hypertension and proteinuria, while blood samples were collected to measure malondialdehyde (MDA) and interleukin-6 (IL-6) levels, as markers of oxidative stress and inflammation, respectively. Statistical analysis was performed using GraphPad Prism 10.2. Results: Selenium improved L-NAME-induced hypertension (Mean BP 107.63±5.22 mmHg vs 140.9±8.38 mmHg in disease control (DC) and proteinuria (65.5±4.09 vs 140.2±11.85 mg/day in DC) and significantly reduced the inflammatory response (IL-6 23.4±1.06 vs 50.63±3.35 pg/mL in DC) but had little effect on oxidative stress (MDA 0.21±0.02 vs 0.24±0.02 nmol/mL in DC). NAC did not lower BP (Mean BP 129.33±7.96 mmHg) but significantly reduced proteinuria (92.7±6.37mg/day), IL-6 levels (18.24±0.42 pg/mL), and oxidative stress (MDA 0.16±0.01 nmol/mL). Conclusions: These findings suggest that selenium and NAC play distinct protective roles in the pathophysiology of preeclampsia, potentially offering synergistic effects for cardiovascular and kidney health in hypertensive pregnancies.</div> Gestational hypertension, Inflammation, Interleukin-6, Oxidative stress, Preeclampsia, Proteinuria. 495 506 http://rbmb.net/browse.php?a_code=A-10-1688-1&slc_lang=en&sid=1 Geethika Yelleti 100319475328460021636 100319475328460021636 No Department of Biochemistry, Kasturba Medical college, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India, 576104. Annayya Rao Aroor 100319475328460021637 100319475328460021637 No Department of Medicine, University of Missouri, Columbia Missouri MO, USA 65212. Revathi Panduranga Shenoy 100319475328460021638 100319475328460021638 No Department of Biochemistry, Kasturba Medical college, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India, 576104. Abhibroto Karmakar 100319475328460021639 100319475328460021639 No Department of General Medicine, Kasturba medical College, Manipal Academy of Higher Education, Manipal, India. Ashwini Rao 100319475328460021640 100319475328460021640 No Department of Biochemistry, Kasturba Medical college, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India, 576104. N Cheryl Rhea Lewis 100319475328460021641 100319475328460021641 No Department of Pharmacology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India, 576104. Arjun Asok 100319475328460021642 100319475328460021642 No Department of Biochemistry, Kasturba Medical college, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India, 576104. Nihaal Maripini 100319475328460021643 100319475328460021643 No Department of Biochemistry, Kasturba Medical college, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India, 576104. Monalisa Biswas 100319475328460021644 100319475328460021644 No Department of Biochemistry, Kasturba Medical college, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India, 576104. Veena Varier 100319475328460021645 100319475328460021645 No Department of Pathology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India, 576104. Arpita Roy 100319475328460021646 100319475328460021646 No Department of Pharmacology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India, 576104. Varashree Bolar Suryakanth varashree.bs@manipal.edu. 100319475328460021647 100319475328460021647 Yes Department of Biochemistry, Kasturba Medical college, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India, 576104.