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en The Effect of Ziziphora clinopodioides Extract on Prostate Cancer Cell Line DU-145 against Ionizing Radiation Compared to Normal Cell Line HEK-293 زیست شناسی سلولی Cell Biology مقالات اصلی Original Article Background: Our study aims to assess how the combination of Ziziphora clinopodioides (Kakoti) extract and gamma radiation affects the survival of prostate cancer cells (DU-145) and normal kidney cells (HEK-293). In addition, the study seeks to investigate the potential protective effects of this extract against damage caused by radiation. Methods: A Ziziphora extract was prepared using standard extraction techniques. The extract's effective dose for both cell lines was determined using the MTT assay. Three grays (Gy) of gamma radiation was used to simulate radiotherapy conditions. The impact of the extract alone, radiation alone, and their combination on cell viability was assessed. Data were analyzed using SPSS version 25 (IBM, USA), with significance set at P< 0.05. Results: The extract significantly protected HEK-293 cells from radiation-induced damage at lower concentrations (P< 0.05), increasing cell viability by up to 20% compared to radiation alone. Conversely, it enhanced cytotoxicity in DU-145 cells, reducing viability by 30-50% at higher concentrations (P< 0.05). However, at higher radiation doses, the protective effect on normal cells diminished, with viability decreasing by 10-15%. Conclusion: Ziziphora clinopodioides extract demonstrates dose-dependent potential as a protective agent for normal cells and a cytotoxic enhancer for prostate cancer cells under gamma radiation. These findings suggest that optimized dosing could be crucial for its clinical application in radiotherapy. Chemotherapy, Gamma Radiation, Oxidative Stress, Prostate Cancer, Radiotherapy, Ziziphora clinopodioides Extract. 250 257 http://rbmb.net/browse.php?a_code=A-10-1713-1&slc_lang=en&sid=1 Shaghayegh Tafazoli 100319475328460022572 100319475328460022572 No Department of Cellular and Molecular Biology, Islamic Azad University of Mashhad, Mashhad, Iran. Nazanin Akbari 100319475328460022573 100319475328460022573 No Department of Biology, Shahid Beheshti University, Tehran, Iran & Clinical Research Development Unit, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran. Fatemeh Faravani 100319475328460022574 100319475328460022574 No Department of Cellular and Molecular Biology, Islamic Azad University of Mashhad, Mashhad, Iran. Jina KhayatZadeh j.khayatzadeh@mshdiau.ac.ir 100319475328460022575 100319475328460022575 Yes Department of Cellular and Molecular Biology, Islamic Azad University of Mashhad, Mashhad, Iran. Banafsheh Heidari 100319475328460022576 100319475328460022576 No Department of Regenerative Medicine in Wound Healing, Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran. Maral Barzegar-Amini* Aminim1@mums.ac.ir 100319475328460022577 100319475328460022577 No Clinical Research Development Unit, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran & Allergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Seyed Reza Hosseini 100319475328460022578 100319475328460022578 No Department of Biology, Mashhad Branch, Islamic Azad University of Mashhad, Mashhad, Iran.