Site is under construction

en A Simplified Protocol for the Purification of Schwann Cells and Exosome Isolation from C57BL/6 Mice زیست شناسی سلولی Cell Biology مقالات اصلی Original Article <div style="text-align: justify;">Background: The purification of Schwann cells has proven to be a difficult process, with most methods requiring the use of special equipment. However, obtaining a sufficient number and high purity of Schwann cells is an integral aspect in their use for clinical application. Therefore, the aim of this study was to establish a simple and effective protocol for the isolation and purification of Schwann cells from the sciatic nerve of C57BL/6 mice. Furthermore, we aimed to provide a protocol for the isolation of exosomes from these cells. Methods: To purify Schwann cells, we used a combination of in situ nerve pre-degeneration and fetal bovine serum. To determine the most effective method of cell purification, we treated the culture with varying concentrations of fetal bovine serum and examined which concentration provided the highest Schwann cell purity. Exosomes were then isolated from Schwann cells through a process of repeated centrifugation and filtration steps. Results: We were able to increase the purified population of Schwann cells from C57BL/6 mice by reducing the concentration of FBS. The purity of Schwann cells at FBS concentrations of 10%, 5%, and 2% were 93.42%, 91.25%, and 97.83%, respectively. Conclusions: When using a concentration of 2% FBS, we obtained the highest purification yield of Schwann cells. Our protocol does not require special equipment or materials. We have created a protocol that is simple, fast, and safe while providing a high yield of purified Schwann cells. The exosome isolation method described in this paper is an appropriate approach with a high quality and yield.</div> Exosome, Fetal bovine serum, purification, predegenaration, Schwann cells 9 15 http://rbmb.net/browse.php?a_code=A-10-84-1&slc_lang=en&sid=1 Mana Shojapour mana_shojapuor@yahoo.com 10031947532846006334 10031947532846006334 Yes Department of molecular Medicine. School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. Ghasem Mosayebi 10031947532846006335 10031947532846006335 No Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran. Reza Hajihossein rhajihossein@arakmu.ac.ir 10031947532846006336 10031947532846006336 No Department of Parasitology, Arak University of Medical Sciences, Arak, Iran. Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Farshid Noorbakhsh -noorbakhsh@sina.tums.ac.ir 10031947532846006337 10031947532846006337 No Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran Aram Mokarizadeh 10031947532846006338 10031947532846006338 No Department of Immunology, Faculty of Medicine, Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran. Mohammad Hossein Ghahremani mhghahremani@tums.ac.ir 10031947532846006339 10031947532846006339 No Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.