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en Effect of Zinc Oxide Nanoparticles on Hepatic Ischemia-Reperfusion Injury: Role of miR-125b Expression in Possible Underlying Mechanisms زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;">Background: This study examined the protective effects of zinc oxide nanoparticles (ZnO-NPs) on hepatic ischemia-reperfusion injury (HIRI) and their possible underlying mechanisms. Methods: 48 male rats were randomly divided into six &lrm;groups (n=8): the sham group that received intraperitoneal normal saline solution (Sham),&lrm; the HIRI group, the control groups pre-treated with 5 and 10 mg/kg ZnO-NPs for 3 &lrm;consecutive days without surgery (ZnO5) and (ZnO10), &lrm;the HIRI group pre-treated with 5 mg/kg ZnO-NPs&lrm; for 3 consecutive days before surgery (HIRI+ZnO5), &lrm;and the HIRI group pre-treated with 10 mg/kg ZnO-NPs&lrm; for 3 &lrm;consecutive days before surgery (HIRI+ZnO10)&lrm;. One hour after reperfusion, serum and tissue samples were collected for biochemical, molecular and histopathological evaluation. Results: Administration of ZnO-NPs caused significant improvement in the elevated serum concentrations of ALT, AST, TOS and MDA, improved liver histopathology, and increased TNF-α, IL-6, and NF-κB levels in liver tissue compared to HIRI group. In addition, administration of ZnO-NPs increased the expression of miR-125 in liver tissue compared than in the HIRI group. Conclusion: The administration of ZnO-NPs improved the effect on HIRI by enhancing miR-125b expression and suppressing oxidative stress and inflammatory cytokines.</div> Inflammation, Ischemia-Reperfusion Injury, Mir-125b, Nanoparticles, Zinc Oxide. 145 154 http://rbmb.net/browse.php?a_code=A-10-1804-1&slc_lang=en&sid=1 Feryal Savari feryal.savari@gmail.com 100319475328460022340 100319475328460022340 Yes Department of Medical Basic Sciences, Shoushtar Faculty of Medical Sciences, Shoushtar, Iran. Seyed Ali Mard 100319475328460022341 100319475328460022341 No Clinical Sciences Research Institute, Alimentary Tract Research Center, Department of Physiology, The School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Annahita Rezaie 100319475328460022342 100319475328460022342 No Department of Pathobiology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran. Mojtaba Kalantar 100319475328460022343 100319475328460022343 No Department of Occupational Health, Shoushtar Faculty of Medical Sciences, Shoushtar, Iran.