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en C-590T Promoter Polymorphism of the Interleukin (IL)-4 Gene Is Associated with an Increased Susceptibility to Nasal Polyposis زیست شناسی ملکولی Molecular Biology مقالات اصلی Original Article <div style="text-align: justify;">Background: Recent studies have shown interleukin 4 (IL-4) and 5 lipoxygenase (5-LO) to play an important role in development of nasal polyposis. Investigation into the genetic factors associated with allergic and non-allergic nasal polyposis has been examined for more than fifteen years. Despite these efforts, the genetic factors underlying the development of nasal polyposis have yet to be clearly understood. The current study examined the relationship between C-590T promoter polymorphisms of the IL-4 gene and the presence of nasal polyps. Additionally, we examined the levels of 5-LO expression in nasal polyp tissue and its association with the IL-4 promoter gene polymorphisms. Methods: A total of 320 subjects were enrolled in the study, of which 256 were healthy controls and 64 were patients with nasal polyps. The Real-Time PCR HRM-based method was used to determine the genotypes of IL-4 C-590T. The expression of 5-LO within the 64 samples of nasal polyp tissue was determined by immunohistochemical staining to examine the association of 5-LO with the IL-4 C-590T genotype.      Results: Genetic analysis showed a significant difference in the frequencies of the IL-4 polymorphisms at C-590T in patients with nasal polyps as compared with controls (p<0.001). No significant difference was seen in the expression of 5-LO among genotypes in patients with nasal polyps (p=0.139). Conclusions: The results suggest that the inheritance of TT and CT genotypes at the IL-4 C-590T promoter gene is associated with nasal polyps however, there is no association between the expression of 5-LO in nasal polyp tissues and IL-4 C-590T genotypes in patients with nasal polyps.  </div> Gene polymorphism, IL-4, IL-4 C-590T, Nasal polyposis, 5-LO. 129 135 http://rbmb.net/browse.php?a_code=A-10-48-3&slc_lang=en&sid=1 Mojgan Mohammadi mohammadimzh@mums.ac.ir 100319475328460017016 100319475328460017016 No Allergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. & Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Shahriar Dabiri dabiri12@yahoo.com 100319475328460017017 100319475328460017017 No Pathology and Stem Cell Research Center, Department of Pathology, Kerman University of Medical Sciences, Kerman, Iran. Hamid Reza Mollaei hamid2008kmu@gmail.com 100319475328460017018 100319475328460017018 No Department of Microbiology and Virology, Kerman University of medical sciences, Kerman, Iran. Samira Rezaee Jouzdani s.rezaei.esf@gmail.com 100319475328460017019 100319475328460017019 No Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran. & Department of Otorhinolaryngology Head and Neck Surgery, Shafa Hospital, University of Medical Sciences, Kerman, Iran. Maryam Amizadeh ma74il@yahoo.com 100319475328460017020 100319475328460017020 No Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran. & Department of Otorhinolaryngology Head and Neck Surgery, Shafa Hospital, University of Medical Sciences, Kerman, Iran. Jamshid Esmailzadeh dr.esmailzadeh@yahoo.com 100319475328460017021 100319475328460017021 No Department of Otorhinolaryngology Head and Neck Surgery, Shafa Hospital, University of Medical Sciences, Kerman, Iran. Mohammad Reza Baneshi rbaneshi2@gmail.com 100319475328460017022 100319475328460017022 No Modelling in health research centre, institute of future studies in health, Kerman University of medical sciences, Kerman, Iran. Aliasghar Arabi Mianroodi mrarabi@yahoo.com 100319475328460017023 100319475328460017023 Yes Department of Otorhinolaryngology Head and Neck Surgery, Shafa Hospital, University of Medical Sciences, Kerman, Iran. & Clinical Research Unit, Shafa Hospital, Kerman University of Medical Sciences, Kerman, Iran.