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en Determining the Biofilm Forming Gene Profile of Staphylococcus aureus Clinical Isolates via Multiplex Colony PCR Method میکروب شناسی Microbiology مقالات اصلی Original Article <div style="text-align: justify;">Background: Among hospitalized patients, Staphylococcus aureus (S. aureus) infections pose a serious health threat. The present study investigated the frequency of biofilm forming genes among clinical isolates S. aureus and their susceptibility to antibiotics. Methods: The clinical samples were analyzed via standard biochemical assays for identifying different bacterium, which was then confirmed using the multiplex colony PCR method. Those samples identified as S. aureus were examined for the presence of the cna, fnbA, fnbB and pvl genes. The antibiotic susceptibility of the S. aureus isolates was then tested. Results: Using the standard biochemical assay approach, 54 S. aureus strains were identified. However, when using the multiplex PCR method 50 S. aureus strains were identified among the clinical samples. The in vitro biofilm formation assays determined 3 (6%) strains of S. aureus to be strong biofilm forming, 15 (30%) of the isolates were determined to be moderate biofilm forming and, 32 (64%) were determined to be weak biofilm forming. Among the isolated strains, the specific frequencies of the biofilm forming genes were determined to be 31 (62%) for cna, 35 (70%) for fnbA, 13 (26%) for fnbB and 1 (2%) for pvl. In 11 (22%) of the isolated strains fnbA, fnbB and cna genes were all present. All strains were determined to be penicillin, amoxicillin and clavulanic acid resistant.   Conclusions: Due to the increase of the antibiotic resistance in biofilm producing S. aureus strains, rapid identification of antibiotic resistance can help to eliminate the infection effectively.</div> Biofilm, Multiplex colony PCR, Pertussis toxin, Spreading factors, Staphylococcus aureus. 181 188 http://rbmb.net/browse.php?a_code=A-10-137-1&slc_lang=en&sid=1 Mostafa Shahmoradi shahmoradimostafa7@gmail.com 100319475328460017069 100319475328460017069 No Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran. Peyman Faridifar peyman.faridifar64@gmail.com 100319475328460017070 100319475328460017070 No Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran. Reza Shapouri rezashapury@yahoo.com 100319475328460017071 100319475328460017071 No Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran. Seyed Fazlollah Mousavi Mousavi@pastur.ac.ir 100319475328460017072 100319475328460017072 Yes Department of Microbiology, Microbial research center, Pasteur Institute of Iran, Tehran. Mahnaz Ezzedin Mhz.ezn@gmail.com 100319475328460017073 100319475328460017073 No Department of Microbiology, Islamic Azad University, Tehran branch, Tehran, Iran. Bahman Mirzaei b.mirzaei@mazums.ac.ir 100319475328460017074 100319475328460017074 No Department of Microbiology, Islamic Azad University, Tehran branch, Tehran, Iran. & Department of Medical Microbiology and Virology Faculty of Medicine, Mazandaran University of medical sciences, Sari, Iran.