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'); Reports of Biochemistry and Molecular Biology rbmb.net Basic Sciences http://rbmb.net 1 admin 2322-3480 2322-3480 10.61882/rbmb en jalali 1391 7 1 gregorian 2012 10 1 1 1 online 1 fulltext
en Validity of Using Recombinant Melon Profilin, Cuc m 2, for Diagnosis of Melon Allergy ایمنی شناسی Immunology <p style="text-align: justify;"><em><strong>Background:</strong></em> Allergy is a clinical disorder affecting humans worldwide. Allergenic extracts prepared from natural source materials remain heterogeneous in composition and content, but are regularly used for diagnosis and immunotherapy. Recombinant allergens are suitable candidates to use in place of natural allergens; however, the recombinant allergens should be assessed and compared with the natural ones. Cuc m 2 (profilin), one of the most important allergens of melon <em>(Cucumis melo)</em>, has been cloned and was expressed in <em>Escherichia coli (E. coli)</em>. We aimed to evaluate the validity of recombinant Cuc m 2 (rCuc m 2) in the diagnosis of melon allergy and investigate whether rCuc m 2 could be used as a replacement for natural Cuc m 2 (nCuc m 2).</p> <p style="text-align: justify;"><em><strong>Methods:</strong></em> nCuc m 2 was purified by immuno-affinity chromatography and rCuc m 2 was purified by metal-affinity chromatography. SDS-PAGE and western blotting were carried out to evaluate the purification methods. Skin prick tests (SPT), and enzyme immunoassays to determine specific IgE, were performed with the natural and recombinant purified allergens on 53 patients with melon allergy.</p> <p style="text-align: justify;"><em><strong>Results:</strong></em> rCuc m 2 elicited no significantly different responses in skin compared with nCuc m 2. All patients&#39; sera showed similar ODs in ELISAs with natural and recombinant profilin.</p> <p style="text-align: justify;"><em>Conclusion: </em>rCuc m 2 evoked strong immuno-reactivity equivalent to nCuc m 2, and has potential for diagnosis of melon allergy.</p> Allergy, Cuc m 2, Melon, Natural allergen, Recombinant allergen 14 20 http://rbmb.net/browse.php?a_code=A-10-1-3&slc_lang=en&sid=1 Mojtaba Sankian 10031947532846005892 10031947532846005892 No Immunobiochemistry Lab, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran Yaser Bagheri 10031947532846005893 10031947532846005893 No Immunobiochemistry Lab, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran Fatemeh Vahedi 10031947532846005894 10031947532846005894 No Razi Vaccine & Serum Research Institute, Mashhad, Iran Farahzad Jabbari Azad 10031947532846005895 10031947532846005895 No Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Abdol-Reza Varasteh varasteha@mums.ac.ir 10031947532846005896 10031947532846005896 Yes Immunobiochemistry Lab, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran