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'); Reports of Biochemistry and Molecular Biology rbmb.net Basic Sciences http://rbmb.net 1 admin 2322-3480 2322-3480 10.61882/rbmb en jalali 1391 7 1 gregorian 2012 10 1 1 1 online 1 fulltext
en Investigation of DNA Integration into Reproductive Organs Following Intramuscular Injection of DNA in Mice بیوشیمی Biochemistry <p style="text-align: justify;"><em><strong>Background:</strong></em> DNA immunization with plasmid DNA encoding bacterial, viral, parasitic, and tumor antigens has been reported to trigger protective immunity. The use of plasmid DNA vaccinations against many diseases has produced promising results in animal and human clinical trials; however, safety concerns about the use of DNA vaccines exist, such as the possibility of integration into the host genome, and elicitation of adverse immune responses.</p> <p style="text-align: justify;"><em><strong>Methods:</strong></em> In this study, we examined the potential integration and bio-distribution of pcDNA3.1+PA, a new vaccine candidate with GenBank accession # EF550208, encoding the PA63 gene, in reproductive organs of mice; ovaries and uterus in female, and testis in male. Animals of both sexes were injected intramuscularly with pcDNA3.1+PA. Host genome integration and tissue distribution were examined using PCR and RT-PCR two times monthly for six months.</p> <p style="text-align: justify;"><strong><em>Results:</em></strong> RT-PCR confirmed that pcDNA3.1+PA was not integrated into the host genome and did not enter reproductive organs.</p> <p style="text-align: justify;"><em><strong>Conclusions:</strong></em> This finding has important implications for the use of pcDNA3.1+PA plasmid as a vaccine and opens new perspectives in the DNA vaccine area.</p> DNA, Intramuscular injection, Integration, Mice, Reproductive organs 21 24 http://rbmb.net/browse.php?a_code=A-10-1-4&slc_lang=en&sid=1 Fatemeh Vahedi vahedif@yahoo.com 10031947532846005897 10031947532846005897 Yes Razi Vaccine & Serum Research Institute, Mashhad, Iran Najmeh Nazari 10031947532846005898 10031947532846005898 No Biochemistry Department, Payam-e-Noor University, Mashhad, Iran Shirin Arbabi 10031947532846005899 10031947532846005899 No Biochemistry Department, Payam-e-Noor University, Mashhad, Iran Yasser Peymanfar 10031947532846005900 10031947532846005900 No Razi Vaccine & Serum Research Institute, Mashhad, Iran