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'); Reports of Biochemistry and Molecular Biology rbmb.net Basic Sciences http://rbmb.net 1 admin 2322-3480 2322-3480 10.61882/rbmb en jalali 1391 7 1 gregorian 2012 10 1 1 1 online 1 fulltext
en Decreased Levels of Soluble Toll-Like Receptor 2 in Patients with Asthma زیست شناسی ملکولی Molecular Biology <p style="text-align: justify;"><strong><em>Background:</em></strong> Recently, reports have indicated a role for the membrane form of Toll-like Receptor 2 (TLR2) in asthma pathogenesis. In this study we examined soluble TLR2 levels in serum and sputum of asthmatic and healthy subjects.</p> <p style="text-align: justify;"><em><strong>Methods:</strong></em> Serum and sputum samples were obtained from 33 asthmatic and 19 healthy subjects. The asthmatics were classified into four groups according to the Global Initiative for Asthma. A sandwich ELISA was developed to measure soluble TLR2 (sTLR2) in serum and sputum. TLR2 mRNA expression was determined by semi-quantitative RT-PCR of all sputum samples.</p> <p style="text-align: justify;"><em><strong>Results:</strong></em> The mean sTLR2 levels from serum and sputum of asthmatics were significantly lower than those from healthy subjects. Moreover, sTLR2 concentration decreased concomitantly with asthma severity. The differences observed, however, were not statistically significant. TLR2/GAPDH mRNA of sputum leukocytes was also significantly lower in asthmatics than in healthy subjects.</p> <p style="text-align: justify;"><em><strong>Conclusion:</strong></em> This study demonstrated for the first time thatsTLR2 levels are lower in serum and sputum samples from asthmatic than from healthy subjects, and this could be an indicator of TLR2 expression. We also found that sTLR2 concentration in serum decreased concomitantly with an increase of asthma severity clinical score.</p> Asthma, Expression, Soluble Toll-like receptor, TLR2 mRNA 30 36 http://rbmb.net/browse.php?a_code=A-10-1-6&slc_lang=en&sid=1 Mohsen Tehrani 10031947532846005907 10031947532846005907 No Immunobiochemistry lab, Immunology Research Center, Bu-Ali Research Institute, Mashhad, Iran Abdol-Reza Varasteh 10031947532846005908 10031947532846005908 No Immunobiochemistry lab, Immunology Research Center, Bu-Ali Research Institute, Mashhad, Iran Mohammad Reza Khakzad 10031947532846005909 10031947532846005909 No Zakaria Research Center, Azad University of Mashhad, Mashhad, Iran Majid Mirsadraee 10031947532846005910 10031947532846005910 No Zakaria Research Center, Azad University of Mashhad, Mashhad, Iran Mojtaba Sankian Sankianm@mums.ac.ir 10031947532846005911 10031947532846005911 Yes Immunobiochemistry lab, Immunology Research Center, Bu-Ali Research Institute, Mashhad, Iran